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홈 > 제품정보 > 시약 > 해외 공식 대리점 > [CSB-E13000h] Human Interleukin 33(IL-33) ELISA Kit
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[CSB-E13000h] Human Interleukin 33(IL-33) ELISA Kit

제품코드 : CSB-E13000h

제품정보 : Human Interleukin 33(IL-33)

  • Sample Types: serum, plasma, urine
  • Detect Range: 15.6 pg/mL-1000 pg/mL
  • Sensitivity: 3.9 pg/mL

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  • 전화번호
    2292-8870

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Product Details

Description
The human IL-33 ELISA Kit is engineered for accurate measurement of human IL-33 levels from samples including serum, plasma, or urine. It uses the Sandwich-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the IL-33 content in the sample. The color intensity is positively correlated with IL-33 content in the sample. The IL-33 concentration can be calculated according to the standard curve. This kit is tested with high sensitivity, strong specificity, good linearity, high precision and recovery, as well as lot-to-lot consistency. IL-33 is constitutively ex...Read more
Target Nameinterleukin 33
Alternative NamesC9orf26 ELISA Kit; CHROMOSOME 9 OPEN READING FRAME 26 ELISA Kit; DKFZp586H0523 ELISA Kit; DVS27 ELISA Kit; DVS27 related protein ELISA Kit; IL 1F11 ELISA Kit; IL 33 ELISA Kit; IL-1F11 ELISA Kit; IL-33 ELISA Kit; IL1F11 ELISA Kit; IL33 ELISA Kit; IL33_HUMAN ELISA Kit; Interleukin 1 family member 11 ELISA Kit; Interleukin 33 ELISA Kit; INTERLEUKIN 33 NFHEV ELISA Kit; Interleukin 33 precursor ELISA Kit; Interleukin-1 family member 11 ELISA Kit; Interleukin-33 (109-270) ELISA Kit; Interleukin33 ELISA Kit; NF HEV ELISA Kit; NF-HEV ELISA Kit; NFEHEV ELISA Kit; NFHEV ELISA Kit; Nuclear factor for high endothelial venules ELISA Kit; Nuclear factor from high endothelial venules ELISA Kit; OTTHUMP00000021041 ELISA Kit; RP11 575C20.2 ELISA Kit
AbbreviationIL33
Uniprot No.O95760
SpeciesHomo sapiens (Human)
Sample Typesserum, plasma, urine
Detection Range15.6 pg/mL-1000 pg/mL
Sensitivity3.9 pg/mL
Assay Time1-5h
Sample Volume50-100ul
Detection Wavelength450 nm
Research AreaImmunology
Assay Principlequantitative
MeasurementSandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human IL-33 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　SampleSerum(n=4)
1:1Average %89
Range %80-94
1:2Average %96
Range %90-99
1:4Average %106
Range %92-115
1:8Average %95
Range %86-100
Recovery
The recovery of human IL-33 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5)9589-99
EDTA plasma (n=4)9790-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10001.8941.7521.8231.728
5001.2751.1861.2311.136
2500.7340.6980.7160.621
1250.4210.4090.4150.320
62.50.2450.2390.2420.147
31.20.1650.1590.1620.067
15.60.1120.1090.1110.016
00.0950.0940.095　
ELISA Data AnalysisWatch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs		ELISA kit FAQs
StorageStore at 2-8°C. Please refer to protocol.
Lead Time3-5 working days

Citations

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