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홈 > 제품정보 > 시약 > 해외 공식 대리점 > [CSB-E13660m] Mouse Interleukin 31(IL-31) ELISA Kit
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[CSB-E13660m] Mouse Interleukin 31(IL-31) ELISA Kit

제품코드 : CSB-E13660m

제품정보 : Mouse Interleukin 31(IL-31)

  • Sample Types: serum, plasma, cell culture supernates, tissue homogenates
  • Detect Range: 31.25 pg/mL-2000 pg/mL
  • Sensitivity: 7.81 pg/mL

제품문의

  • 전화번호
    2292-8870

제품상세

Product Details

Description

This Mouse IL31 ELISA Kit was designed for the quantitative measurement of Mouse IL31 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL .

Target Nameinterleukin 31
Alternative NamesIl31Interleukin-31 ELISA Kit; IL-31 ELISA Kit
AbbreviationIL31
Uniprot No.Q6EAL8
SpeciesMus musculus (Mouse)
Sample Typesserum, plasma, cell culture supernates, tissue homogenates
Detection Range31.25 pg/mL-2000 pg/mL
Sensitivity7.81 pg/mL
Assay Time1-5h
Sample Volume50-100ul
Detection Wavelength450 nm
Research AreaImmunology
Assay Principlequantitative
MeasurementSandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-31 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　SampleSerum(n=4)
1:1Average %90
Range %82-99
1:2Average %97
Range %87-105
1:4Average %95
Range %88-100
1:8Average %90
Range %82-98
Recovery
The recovery of mouse IL-31 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5)9793-104
EDTA plasma (n=4)9990-106
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
20002.8862.8462.8662.702
10002.3472.3362.3422.178
5001.5591.6021.5811.417
2500.8970.8780.8880.724
1250.5320.5570.5450.381
62.50.3660.3740.3700.206
31.250.2620.2450.2540.090
00.1620.1650.164　
ELISA Data AnalysisWatch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs		ELISA kit FAQs
StorageStore at 2-8°C. Please refer to protocol.
Lead Time3-5 working days

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