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홈 > 제품정보 > 시약 > 해외 공식 대리점 > [CSB-E13296h] Human Interleukin 28B(IL-28B) ELISA kit
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[CSB-E13296h] Human Interleukin 28B(IL-28B) ELISA kit

제품코드 : CSB-E13296h

제품정보 : Human Interleukin 28B(IL-28B)

  • Sample Types: serum, plasma, cell culture supernats, tissue homogenates
  • Detect Range: 1.56 pg/mL-100 pg/mL
  • Sensitivity: 0.39 pg/mL

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  • 전화번호
    2292-8870

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Product Details

Description

This Human IL-28B ELISA Kit was designed for the quantitative measurement of Human IL-28B protein in serum, plasma, cell culture supernats, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 pg/mL-100 pg/mL and the sensitivity is 0.39 pg/mL .

Target NameInterleukin 28B(IL-28B)
Alternative NamesCytokine ZCYTO22 ELISA Kit; IFN-lambda-3 ELISA Kit; IFN-lambda-4 ELISA Kit; IL-28B ELISA Kit; IL-28C ELISA Kit; IL28B ELISA Kit; IL28B_HUMAN ELISA Kit; IL28C ELISA Kit; Interferon lambda-3 ELISA Kit; Interferon lambda-4 ELISA Kit; interleukin 28B (interferon; lambda 3) ELISA Kit; Interleukin 28C ELISA Kit; Interleukin-28B ELISA Kit; Interleukin-28C ELISA Kit
AbbreviationIL-28B
Uniprot No.Q8IZI9
SpeciesHomo sapiens (Human)
Sample Typesserum, plasma, cell culture supernats, tissue homogenates
Detection Range1.56 pg/mL-100 pg/mL
Sensitivity0.39 pg/mL
Assay Time1-5h
Sample Volume50-100ul
Detection Wavelength450 nm
Research AreaImmunology
Assay Principlequantitative
MeasurementSandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human IL-28B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　SampleSerum(n=4)
1:1Average %87
Range %82-92
1:2Average %96
Range %90-100
1:4Average %100
Range %95-105
1:8Average %96
Range %93-99
Recovery
The recovery of human IL-28B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5)9490-98
EDTA plasma (n=4)10095-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
1002.2242.2392.2322.047
501.5091.5341.5221.337
251.0231.0361.0300.845
12.50.6880.6990.6940.509
6.250.4740.4900.4820.297
3.120.3870.3740.3810.196
1.560.2810.2970.2890.104
00.1840.1860.185　
ELISA Data AnalysisWatch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs		ELISA kit FAQs
StorageStore at 2-8°C. Please refer to protocol.
Lead Time3-5 working days

Citations