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홈 > 제품정보 > 시약 > 해외 공식 대리점 > [CSB-E08466m] Mouse Interleukin 27,IL-27 ELISA Kit
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[CSB-E08466m] Mouse Interleukin 27,IL-27 ELISA Kit

제품코드 : CSB-E08466m

제품정보 : Mouse Interleukin 27,IL-27

  • Sample Types: serum, plasma, cell culture supernates, tissue homogenates
  • Detect Range: 31.25 pg/mL-2000 pg/mL
  • Sensitivity: 7.81 pg/mL

제품문의

  • 전화번호
    2292-8870

제품상세

Product Details

Description

This Mouse IL27 ELISA Kit was designed for the quantitative measurement of Mouse IL27 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.

Target Nameinterleukin 27
Alternative NamesIl27 ELISA Kit; Il27aInterleukin-27 subunit alpha ELISA Kit; IL-27 subunit alpha ELISA Kit; IL-27-A ELISA Kit; IL27-A ELISA Kit; p28 ELISA Kit
AbbreviationIL27
Uniprot No.Q8K3I6
SpeciesMus musculus (Mouse)
Sample Typesserum, plasma, cell culture supernates, tissue homogenates
Detection Range31.25 pg/mL-2000 pg/mL
Sensitivity7.81 pg/mL
Assay Time1-5h
Sample Volume50-100ul
Detection Wavelength450 nm
Research AreaImmunology
Assay Principlequantitative
MeasurementSandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-27 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　SampleSerum(n=4)
1:1Average %89
Range %85-93
1:2Average %106
Range %103-109
1:4Average %83
Range %80-85
1:8Average %98
Range %94-102
Recovery
The recovery of mouse IL-27 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5)10095-105
EDTA plasma (n=4)9489-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
20002.5842.6312.6082.471
10001.9791.9981.9891.852
5001.3321.3011.3171.180
2500.7820.7740.7780.641
1250.4850.4680.4770.340
62.50.2630.2780.2710.134
31.250.2060.2140.2100.073
00.1380.1350.137　
ELISA Data AnalysisWatch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs		ELISA kit FAQs
StorageStore at 2-8°C. Please refer to protocol.
Lead Time3-5 working days

Citations

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