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홈 > 제품정보 > 시약 > 해외 공식 대리점 > [CSB-E08466m] Mouse Interleukin 27,IL-27 ELISA Kit

[CSB-E08466m] Mouse Interleukin 27,IL-27 ELISA Kit

제품코드 : CSB-E08466m

제품정보 : Mouse Interleukin 27,IL-27

Sample Types: serum, plasma, cell culture supernates, tissue homogenates
Detect Range: 31.25 pg/mL-2000 pg/mL
Sensitivity: 7.81 pg/mL

제품문의

전화번호

2292-8870

이메일

dreamcell@dreamcell.co.kr

제품상세

Product Details

Description

This Mouse IL27 ELISA Kit was designed for the quantitative measurement of Mouse IL27 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.

Target Name

interleukin 27

Alternative Names

Il27 ELISA Kit; Il27aInterleukin-27 subunit alpha ELISA Kit; IL-27 subunit alpha ELISA Kit; IL-27-A ELISA Kit; IL27-A ELISA Kit; p28 ELISA Kit

Abbreviation

IL27

Uniprot No.

Q8K3I6

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

31.25 pg/mL-2000 pg/mL

Sensitivity

7.81 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-27 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	85-93
1:2	Average %	106
	Range %	103-109
1:4	Average %	83
	Range %	80-85
1:8	Average %	98
	Range %	94-102

Recovery

The recovery of mouse IL-27 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	95-105
EDTA plasma (n=4)	94	89-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.584	2.631	2.608	2.471
1000	1.979	1.998	1.989	1.852
500	1.332	1.301	1.317	1.180
250	0.782	0.774	0.778	0.641
125	0.485	0.468	0.477	0.340
62.5	0.263	0.278	0.271	0.134
31.25	0.206	0.214	0.210	0.073
0	0.138	0.135	0.137

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Citations

Anti-inflammatory Activity of Caspian Cobra (Naja naja oxiana) Snake Venom on the Serum Level of Interleukin-27 and Histopathological Changes in Myelin Oligodendrocyte Glycoprotein-experimental Autoimmune Encephalomyelitis-induced Mice L. Mohammadnejad,Archives of Razi Institute,2020
Combinatory FK506 and Minocycline Treatment Alleviates Prion-Induced Neurodegenerative Events via Caspase-Mediated MAPK-NRF2 Pathway Shah SZA, et al,Renvironmental science and pollution research,2019
Immuno-regulatory function of IL-27 and TGFß1 in cardiac allograft transplantation. Laetitia Le Texier et al,Transplantation,2012

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