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Product Details

[CSB-E11715h] Human Interleukin 25,IL-25 ELISA kit

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홈 > 제품정보 > 시약 > 해외 공식 대리점 > [CSB-E11715h] Human Interleukin 25,IL-25 ELISA kit

제품코드 : CSB-E11715h

제품정보 : Human Interleukin 25,IL-25

Sample Types: serum, cell culturesupernates, urine, tissue homogenates
Detect Range: 62.5 pg/mL-4000 pg/mL
Sensitivity: 15.6 pg/mL

전화번호

2292-8870

이메일

dreamcell@dreamcell.co.kr

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Description

The human IL-25 ELISA kit is suitable for the quantitative determination of human IL-25 in different sample types, including serum, cell culturesupernates, urine, and tissue homogenates. This assay employs the bi-antibody sandwich technique and enzyme-substrate chromogenic reaction to quantify antigen levels in the sample. The amount of synthesized colored products is positively related to the analyte of interest in the sample. IL-25, generated by epithelial cells along with numerous innate immune cells, plays an important role in increasing Th2 cytokine synthesis. Once secreted, IL-25 can bin...Read more

Target Name

interleukin 25

Alternative Names

IL 17E ELISA Kit; IL 25 ELISA Kit; IL-17E ELISA Kit; IL-25 ELISA Kit; IL17e ELISA Kit; IL25 ELISA Kit; IL25_HUMAN ELISA Kit; Interleukin 17E ELISA Kit; Interleukin 25 ELISA Kit; Interleukin-17E ELISA Kit; Interleukin-25 ELISA Kit; Interleukin17E ELISA Kit; Interleukin25 ELISA Kit; OTTHUMP00000027946 ELISA Kit; OTTHUMP00000246238 ELISA Kit; UNQ3120/PRO10272 ELISA Kit

Abbreviation

IL25

Uniprot No.

Q9H293

Species

Homo sapiens (Human)

Sample Types

serum, cell culturesupernates, urine, tissue homogenates

Detection Range

62.5 pg/mL-4000 pg/mL

Sensitivity

15.6 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IL-25 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	87-98
1:2	Average %	97
	Range %	90-103
1:4	Average %	97
	Range %	90-106
1:8	Average %	98
	Range %	94-103

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	2.892	2.752	2.822	2.711
2000	2.299	2.179	2.239	2.128
1000	1.645	1.599	1.622	1.511
500	0.982	1.002	0.992	0.881
250	0.561	0.602	0.582	0.471
125	0.350	0.366	0.358	0.247
62.5	0.268	0.275	0.272	0.161
0	0.109	0.112	0.111