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[CSB-E08463m] Mouse Interleukin 23,IL-23 ELISA Kit

제품코드 : CSB-E08463m

제품정보 : Mouse Interleukin 23,IL-23

Sample Types: serum, plasma, tissue homogenates
Detect Range: 1.56 pg/mL-100 pg/mL
Sensitivity: 1.95 pg/mL

제품문의

전화번호

2292-8870

이메일

dreamcell@dreamcell.co.kr

제품상세

Product Details

Description

This Mouse IL-23 ELISA Kit was designed for the quantitative measurement of Mouse IL-23 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 pg/mL-100 pg/mL and the sensitivity is 1.95 pg/mL.

Target Name

Interleukin 23,IL-23

Alternative Names

N/A

Abbreviation

IL-23

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates

Detection Range

1.56 pg/mL-100 pg/mL

Sensitivity

1.95 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-23 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	81-92
1:2	Average %	95
	Range %	89-100
1:4	Average %	93
	Range %	84-97
1:8	Average %	106
	Range %	97-110

Recovery

The recovery of mouse IL-23 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	94-108
EDTA plasma (n=4)	101	94-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
500	2.868	2.689	2.779	2.654
250	1.519	1.586	1.553	1.428
125	0.746	0.700	0.723	0.598
62.5	0.434	0.456	0.445	0.320
31.2	0.314	0.301	0.308	0.183
15.6	0.207	0.202	0.205	0.080
7.8	0.153	0.159	0.156	0.031
0	0.127	0.123	0.125

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Citations

IFN-r induced by IL-12 administration prevents diabetes by inhibiting pathogenic IL-17 production in NOD mice Jun Zhang et al,Journal of Autoimmunity,2012

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