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> 제품정보 > 시약 > 해외 공식 대리점 > [CSB-E08461h] Human Interleukin 23,IL-23 ELISA Kit
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[CSB-E08461h] Human Interleukin 23,IL-23 ELISA Kit

제품코드 : CSB-E08461h

제품정보 : Human Interleukin 23,IL-23

  • Sample Types: serum, plasma, tissue homogenates
  • Detect Range: 6.25 pg/mL-400 pg/mL
  • Sensitivity: 1.56 pg/mL

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  • 전화번호
    2292-8870

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Product Details

Description
The human IL-23 ELISA Kit is engineered for accurate measurement of human IL-23 levels from samples including serum, plasma, or tissue homogenates. It uses the Sandwich-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the IL-23 content in the sample. The color intensity is positively correlated with IL-23 content in the sample. This kit has been validated against standards of sensitivity, specificity, precision, linearity, recovery, and lot-to-lot consistency. IL-23 is a heterodimeric cytokine consisting of an IL-12p40 subunit and an IL-23-specific p19 s...Read more
Target NameInterleukin 23,IL-23
Alternative NamesN/A
AbbreviationIL-23
SpeciesHomo sapiens (Human)
Sample Typesserum, plasma, tissue homogenates
Detection Range6.25 pg/mL-400 pg/mL
Sensitivity1.56 pg/mL
Assay Time1-5h
Sample Volume50-100ul
Detection Wavelength450 nm
Research AreaImmunology
Assay Principlequantitative
MeasurementSandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%   
Three samples of known concentration were tested twenty times on one plate to assess. 
Inter-assay Precision (Precision between assays): CV%<10%   
Three samples of known concentration were tested in twenty assays to assess.  
       
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human IL-23 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4) 
1:1Average %92 
Range %88-96 
1:2Average %98 
Range %92-106 
1:4Average %94 
Range %90-98 
1:8Average %95 
Range %89-99 
Recovery
The recovery of human IL-23 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange 
Serum (n=5)8982-96 
EDTA plasma (n=4)9389-99 
       
       
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected 
4002.6082.5072.5582.415 
2001.8651.7641.8151.672 
1001.2071.1861.1971.054 
500.8010.7900.7960.653 
250.4370.4480.4430.300 
12.50.3010.3120.3070.164 
6.250.2280.2340.2310.088 
00.1420.1430.143  
ELISA Data AnalysisWatch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
StorageStore at 2-8°C. Please refer to protocol.
Lead Time3-5 working days

Citations