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Product Details

[CSB-E13513m] Mouse Interleukin 22(IL-22) ELISA Kit

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제품상세

홈 > 제품정보 > 시약 > 해외 공식 대리점 > [CSB-E13513m] Mouse Interleukin 22(IL-22) ELISA Kit

제품코드 : CSB-E13513m

제품정보 : Mouse Interleukin 22(IL-22)

Sample Types: serum, plasma, cell culture supernates, tissue homogenates
Detect Range: 0.312 ng/mL-20 ng/mL
Sensitivity: 0.078 ng/mL

전화번호

2292-8870

이메일

dreamcell@dreamcell.co.kr

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Description

This Mouse IL22 ELISA Kit was designed for the quantitative measurement of Mouse IL22 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

Target Name

interleukin 22

Alternative Names

Il22 ELISA Kit; Il22a ELISA Kit; Iltif ELISA Kit; IltifaInterleukin-22 ELISA Kit; IL-22 ELISA Kit; IL-10-related T-cell-derived-inducible factor ELISA Kit; IL-TIF ELISA Kit; IL-TIF alpha ELISA Kit; Interleukin-22a ELISA Kit; IL-22a ELISA Kit

Abbreviation

IL22

Uniprot No.

Q9JJY9

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

0.312 ng/mL-20 ng/mL

Sensitivity

0.078 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-22 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	106
	Range %	101-113
1:2	Average %	97
	Range %	92-103
1:4	Average %	90
	Range %	85-94
1:8	Average %	94
	Range %	90-98

Recovery

The recovery of mouse IL-22 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	89	84-93

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.309	2.438	2.374	2.157
10	1.628	1.729	1.679	1.462
5	1.193	1.221	1.207	0.990
2.5	0.824	0.805	0.815	0.598
1.25	0.605	0.627	0.616	0.399
0.625	0.462	0.450	0.456	0.239
0.312	0.327	0.312	0.320	0.103
0	0.220	0.214	0.217