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> 제품정보 > 시약 > 해외 공식 대리점 > [CSB-E11843r] Rat Interleukin 21,IL-21 ELISA Kit
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[CSB-E11843r] Rat Interleukin 21,IL-21 ELISA Kit

제품코드 : CSB-E11843r

제품정보 : Rat Interleukin 21,IL-21

  • Sample Types: serum, plasma, tissue homogenates
  • Detect Range: 15.6 pg/mL-1000 pg/mL
  • Sensitivity: 3.9 pg/mL

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  • 전화번호
    2292-8870

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Product Details

Description

This Rat IL21 ELISA Kit was designed for the quantitative measurement of Rat IL21 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

Target Nameinterleukin 21
Alternative NamesIl21Interleukin-21 ELISA Kit; IL-21 ELISA Kit
AbbreviationIL21
Uniprot No.A3QPB9
SpeciesRattus norvegicus (Rat)
Sample Typesserum, plasma, tissue homogenates
Detection Range15.6 pg/mL-1000 pg/mL
Sensitivity3.9 pg/mL
Assay Time1-5h
Sample Volume50-100ul
Detection Wavelength450 nm
Research AreaImmunology
Assay Principlequantitative
MeasurementSandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-21 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %91
Range %85-96
1:2Average %89
Range %84-95
1:4Average %103
Range %99-108
1:8Average %99
Range %94-105
Recovery
The recovery of rat IL-21 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5)9792-101
EDTA plasma (n=4)9288-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.6012.5442.5732.456
5001.9211.9521.9371.820
2501.1981.1221.1601.043
1250.7350.7090.7220.605
62.50.4250.4590.4420.325
31.20.2720.2570.2650.148
15.60.1810.1720.1770.060
00.1160.1180.117
ELISA Data AnalysisWatch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
StorageStore at 2-8°C. Please refer to protocol.
Lead Time3-5 working days