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Product Details

[CSB-E04610r] Rat Interleukin 18,IL-18 Elisa Kit

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홈 > 제품정보 > 시약 > 해외 공식 대리점 > [CSB-E04610r] Rat Interleukin 18,IL-18 Elisa Kit

제품코드 : CSB-E04610r

제품정보 : Rat Interleukin 18,IL-18

Sample Types: serum, plasma, cell culture supernates
Detect Range: 0.156 ng/mL-10 ng/mL
Sensitivity: 0.039 ng/mL

전화번호

2292-8870

이메일

dreamcell@dreamcell.co.kr

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Description

The product CSB-E04610r is a ready-to-use microwell, strip plate ELISA kit for quantitatively analyzing the presence of the IL-18 in different biological samples, including serum, plasma, and tissue homogenates. This IL-18 ELISA kit specifically recognizes the rat IL-18 in the sample. It adopts the Sandwich ELISA technique in conjunction with antibody-antigen specific binding and an enzyme colorimetric reaction to indicate the presence of rat IL-18 and quantitatively measure the concentration of IL-18 in the samples. IL-18 is a proinflammatory cytokine that primarily functions to augment the g...Read more

Target Name

interleukin 18 (interferon-gamma-inducing factor)

Alternative Names

Il18 ELISA Kit; Igif ELISA Kit; Interleukin-18 ELISA Kit; IL-18 ELISA Kit; Interferon gamma-inducing factor ELISA Kit; IFN-gamma-inducing factor ELISA Kit; Interleukin-1 gamma ELISA Kit; IL-1 gamma ELISA Kit

Abbreviation

IL18

Uniprot No.

P97636

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, cell culture supernates

Detection Range

0.156 ng/mL-10 ng/mL

Sensitivity

0.039 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	90-102
1:2	Average %	89
	Range %	84-95
1:4	Average %	100
	Range %	95-106
1:8	Average %	97
	Range %	92-104

Recovery

The recovery of rat IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	93-96
EDTA plasma (n=4)	102	98-107

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.357	2.387	2.372	2.218
5	1.686	1.699	1.693	1.539
2.5	1.064	1.015	1.040	0.886
1.25	0.687	0.654	0.671	0.517
0.625	0.382	0.398	0.390	0.236
0.312	0.289	0.270	0.280	0.126
0.156	0.221	0.235	0.228	0.074
0	0.151	0.157	0.154