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홈 > 제품정보 > 시약 > 해외 공식 대리점 > [CSB-EL011601RA] Rat Interleukin-17F(IL17F) ELISA kit
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[CSB-EL011601RA] Rat Interleukin-17F(IL17F) ELISA kit

제품코드 : CSB-EL011601RA

제품정보 : Rat Interleukin-17F(IL17F)

  • Sample Types: serum, plasma, tissue homogenates
  • Detect Range: 15.6 pg/mL-1000 pg/mL
  • Sensitivity: 3.9 pg/mL

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  • 전화번호
    2292-8870

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Product Details

Description

This Rat IL17F ELISA Kit was designed for the quantitative measurement of Rat IL17F protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

Target Nameinterleukin 17F
Alternative NamesIl17fInterleukin-17F ELISA Kit; IL-17F ELISA Kit
AbbreviationIL17F
Uniprot No.Q5BJ95
SpeciesRattus norvegicus (Rat)
Sample Typesserum, plasma, tissue homogenates
Detection Range15.6 pg/mL-1000 pg/mL
Sensitivity3.9 pg/mL
Assay Time1-5h
Sample Volume50-100ul
Detection Wavelength450 nm
Research AreaImmunology
Assay Principlequantitative
MeasurementSandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat IL17F in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　SampleSerum(n=4)
1:1Average %95
Range %91-99
1:2Average %83
Range %80-86
1:4Average %95
Range %80-90
1:8Average %102
Range %98-106
Recovery
The recovery of rat IL17F spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5)8783-90
EDTA plasma (n=4)104100-108
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.8062.7692.7882.693
5002.3042.4012.3532.258
2501.5351.6051.5701.475
1250.6800.6980.6890.594
62.50.3430.3510.3470.252
31.20.2020.1990.2010.106
15.60.1270.1290.1280.033
00.0950.0940.095　
ELISA Data AnalysisWatch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs		ELISA kit FAQs
StorageStore at 2-8°C. Please refer to protocol.
Lead Time3-5 working days

Citations

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