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홈 > 제품정보 > 시약 > 해외 공식 대리점 > [CSB-EL011601MO] Mouse Interleukin-17F(IL17F) ELISA kit

[CSB-EL011601MO] Mouse Interleukin-17F(IL17F) ELISA kit

제품코드 : CSB-EL011601MO

제품정보 : Mouse Interleukin-17F(IL17F)

Sample Types: serum, plasma, tissue homogenates
Detect Range: 31.25 pg/mL-2000 pg/mL
Sensitivity: 7.81 pg/mL

제품문의

전화번호

2292-8870

이메일

dreamcell@dreamcell.co.kr

제품상세

Product Details

Description

This Mouse IL17F ELISA Kit was designed for the quantitative measurement of Mouse IL17F protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.

Alternative Names

Il17fInterleukin-17F ELISA Kit; IL-17F ELISA Kit

Abbreviation

IL17F

Uniprot No.

Q7TNI7

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates

Detection Range

31.25 pg/mL-2000 pg/mL

Sensitivity

7.81 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL17F in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	91-103
1:2	Average %	91
	Range %	83-95
1:4	Average %	94
	Range %	86-98
1:8	Average %	95
	Range %	90-100

Recovery

The recovery of mouse IL17F spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	94-101
EDTA plasma (n=4)	101	90-106

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
200	1.796	1.829	1.813	1.597
100	1.346	1.368	1.357	1.141
50	0.930	0.976	0.953	0.737
25	0.712	0.734	0.723	0.507
12.5	0.515	0.527	0.521	0.305
6.25	0.407	0.423	0.415	0.199
3.12	0.311	0.319	0.315	0.099
0	0.218	0.214	0.216

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Citations

CaMK4-dependent phosphorylation of Akt/mTOR underlies Th17 excessive activation in experimental autoimmune prostatitis CS Zhan,FASEB J.,2020
Melatonin alleviates weanling stress in mice: involvement of intestinal microbiota Ren W.et al,J Pineal Res.,2017
Isorhamnetin attenuates collagen-induced arthritis via modulating cytokines and oxidative stress in mice Wang X. et al,Int J Clin Exp Med,2015

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