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[CSB-E15916h] Human Interleukin17F (IL17F) ELISA Kit

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홈 > 제품정보 > 시약 > 해외 공식 대리점 > [CSB-E15916h] Human Interleukin17F (IL17F) ELISA Kit

제품코드 : CSB-E15916h

제품정보 : Human Interleukin17F (IL17F)

Sample Types: serum, plasma, cell culture supernates
Detect Range: 12.5 pg/mL-800 pg/mL
Sensitivity: 3.1 pg/mL

전화번호

2292-8870

이메일

dreamcell@dreamcell.co.kr

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Description

This Human IL17F ELISA Kit was designed for the quantitative measurement of Human IL17F protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 12.5 pg/mL-800 pg/mL and the sensitivity is 3.1 pg/mL .

Target Name

interleukin 17F

Alternative Names

CANDF6 ELISA Kit; Cytokine ML 1 ELISA Kit; Cytokine ML-1 ELISA Kit; IL 17F ELISA Kit; IL 24 ELISA Kit; IL-17F ELISA Kit; IL-24 ELISA Kit; Il17f ELISA Kit; IL17F_HUMAN ELISA Kit; Interleukin 17F ELISA Kit; Interleukin 24 ELISA Kit; Interleukin-17F ELISA Kit; Interleukin-24 ELISA Kit; ML 1 ELISA Kit; ML1 ELISA Kit; Mutant IL 17F ELISA Kit; OTTHUMP00000016602 ELISA Kit

Abbreviation

IL17F

Uniprot No.

Q96PD4

Species

Homo sapiens (Human)

Sample Types

serum, plasma, cell culture supernates

Detection Range

12.5 pg/mL-800 pg/mL

Sensitivity

3.1 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IL17F in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	87-99
1:2	Average %	103
	Range %	99-108
1:4	Average %	80
	Range %	85-96
1:8	Average %	93
	Range %	85-98

Recovery

The recovery of human IL17F spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-96
EDTA plasma (n=4)	89	85-93

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
800	1.850	1.920	1.885	1.795
400	1.342	1.405	1.374	1.284
200	0.834	0.902	0.868	0.778
100	0.557	0.525	0.541	0.451
50	0.325	0.342	0.334	0.244
25	0.253	0.264	0.259	0.169
12.5	0.146	0.150	0.148	0.058
0	0.087	0.092	0.090