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> 제품정보 > 시약 > 해외 공식 대리점 > [CSB-E07453r] Rat Interleukin 15,IL-15 ELISA KIT
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[CSB-E07453r] Rat Interleukin 15,IL-15 ELISA KIT

제품코드 : CSB-E07453r

제품정보 : Rat Interleukin 15,IL-15

  • Sample Types: serum, plasma, tissue homogenates, cell lysates
  • Detect Range: 1.56 pg/mL-100 pg/mL
  • Sensitivity: 0.39 pg/mL

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  • 전화번호
    2292-8870

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Product Details

Description

This Rat IL15 ELISA Kit was designed for the quantitative measurement of Rat IL15 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 1.56 pg/mL-100 pg/mL and the sensitivity is 0.39 pg/mL .

Target Nameinterleukin 15
Alternative NamesIl15 ELISA Kit; Interleukin-15 ELISA Kit; IL-15 ELISA Kit
AbbreviationIL15
Uniprot No.P97604
SpeciesRattus norvegicus (Rat)
Sample Typesserum, plasma, tissue homogenates, cell lysates
Detection Range1.56 pg/mL-100 pg/mL
Sensitivity0.39 pg/mL
Assay Time1-5h
Sample Volume50-100ul
Detection Wavelength450 nm
Research AreaImmunology
Assay Principlequantitative
MeasurementSandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-15 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %96
Range %90-101
1:2Average %93
Range %87-99
1:4Average %87
Range %82-94
1:8Average %100
Range %95-104
Recovery
The recovery of rat IL-15 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5)9691-101
EDTA plasma (n=4)9387-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
1002.5112.5672.5392.383
501.7731.7831.7781.622
251.1061.1541.1300.974
12.50.7040.7250.7150.559
6.250.4150.4310.4230.267
3.120.2870.2970.2920.136
1.560.2140.2310.2230.067
00.1550.1570.156 
ELISA Data AnalysisWatch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
StorageStore at 2-8°C. Please refer to protocol.
Lead Time3-5 working days