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[CSB-E07360m] Mouse interleukin 12,IL-12/P40 ELISA KIT

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홈 > 제품정보 > 시약 > 해외 공식 대리점 > [CSB-E07360m] Mouse interleukin 12,IL-12/P40 ELISA KIT

제품코드 : CSB-E07360m

제품정보 : Mouse interleukin 12,IL-12/P40

Sample Types: serum, plasma, tissue homogenates
Detect Range: 15.6 pg/mL-1000 pg/mL
Sensitivity: 3.9 pg/mL

전화번호

2292-8870

이메일

dreamcell@dreamcell.co.kr

Description

This Mouse IL12B ELISA Kit was designed for the quantitative measurement of Mouse IL12B protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL .

Target Name

interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)

Alternative Names

Il12bInterleukin-12 subunit beta ELISA Kit; IL-12B ELISA Kit; Cytotoxic lymphocyte maturation factor 40 kDa subunit ELISA Kit; CLMF p40 ELISA Kit; IL-12 subunit p40 ELISA Kit

Abbreviation

IL12B

Uniprot No.

P43432

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates

Detection Range

15.6 pg/mL-1000 pg/mL

Sensitivity

3.9 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-12/P40 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	92
	Range %	88-95
1:10	Average %	90
	Range %	85-92
1:20	Average %	89
	Range %	84-96
1:40	Average %	92
	Range %	86-98

Recovery

The recovery of mouse IL-12/P40 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-100
EDTA plasma (n=4)	93	87-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.701	2.852	2.777	2.633
500	2.005	2.076	2.041	1.897
250	1.248	1.316	1.282	1.138
125	0.754	0.704	0.729	0.585
62.5	0.476	0.450	0.463	0.319
31.2	0.311	0.323	0.317	0.173
15.6	0.252	0.259	0.256	0.112
0	0.148	0.139	0.144

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.