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> 제품정보 > 시약 > 해외 공식 대리점 > [CSB-E04599h] Human Interleukin 12,IL-12/P70 ELISA KIT
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[CSB-E04599h] Human Interleukin 12,IL-12/P70 ELISA KIT

제품코드 : CSB-E04599h

제품정보 : Human Interleukin 12,IL-12/P70

  • Sample Types: serum, plasma, cell culture supernates
  • Detect Range: 31.25 pg/ml - 2000pg/ml
  • Sensitivity: 7.8 pg/ml

제품문의

  • 전화번호
    2292-8870

제품상세

Product Details

Description

This Human IL-12/P70 ELISA Kit was designed for the quantitative measurement of Human IL-12/P70 protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/ml-2000 pg/ml and the sensitivity is 7.81 pg/ml.

AbbreviationIL-12/P70
SpeciesHomo sapiens (Human)
Sample Typesserum, plasma, cell culture supernates
Detection Range31.25 pg/ml - 2000pg/ml
Sensitivity7.8 pg/ml
Assay Time1-5h
Sample Volume50-100ul
Detection Wavelength450 nm
Research AreaImmunology
Assay Principlequantitative
MeasurementSandwich
Materials provided
    • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human IL-12 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled IL-12 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
    • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
    • An instruction manual
Materials not provided
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution
ELISA Data AnalysisWatch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
StorageStore at 2-8°C. Please refer to protocol.
Lead Time3-5 working days