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홈 > 제품정보 > 시약 > 해외 공식 대리점 > [CSB-E07294r] Rat Interleukin 9,IL-9 ELISA KIT
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[CSB-E07294r] Rat Interleukin 9,IL-9 ELISA KIT

제품코드 : CSB-E07294r

제품정보 : Rat Interleukin 9,IL-9

  • Sample Types: serum, plasma, cell culture supernates, tissue homogenates
  • Detect Range: 15.6 pg/mL-1000 pg/mL
  • Sensitivity: 3.9 pg/mL

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  • 전화번호
    2292-8870

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Product Details

Description

This Rat IL-9 ELISA Kit was designed for the quantitative measurement of Rat IL-9 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

Target NameInterleukin 9,IL-9
AbbreviationIL-9
Uniprot No.D4A8I9
SpeciesRattus norvegicus (Rat)
Sample Typesserum, plasma, cell culture supernates, tissue homogenates
Detection Range15.6 pg/mL-1000 pg/mL
Sensitivity3.9 pg/mL
Assay Time1-5h
Sample Volume50-100ul
Detection Wavelength450 nm
Research AreaImmunology
Assay Principlequantitative
MeasurementSandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %97
Range %92-103
1:2Average %92
Range %88-96
1:4Average %95
Range %90-99
1:8Average %84
Range %80-88
Recovery
The recovery of rat IL-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5)9085-95
EDTA plasma (n=4)9893-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.4892.3532.4212.322
5001.9542.0762.0151.916
2501.4811.4441.4631.364
1250.8230.8060.8150.716
62.50.4020.4170.4100.311
31.20.2150.2290.2220.123
15.60.1600.1540.1570.058
00.0960.1020.099
ELISA Data AnalysisWatch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs		ELISA kit FAQs
StorageStore at 2-8°C. Please refer to protocol.
Lead Time3-5 working days

Citations