| Description | This Human IL4 ELISA Kit was designed for the quantitative measurement of Human IL4 protein in serum, tissue homogenates, cell culture supernates, saliva. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL. |
| Alternative Names | B cell growth factor 1 ELISA Kit; B cell IgG differentiation factor ELISA Kit; B Cell Stimulatory Factor 1 ELISA Kit; B-cell stimulatory factor 1 ELISA Kit; BCGF 1 ELISA Kit; BCGF1 ELISA Kit; Binetrakin ELISA Kit; BSF-1 ELISA Kit; BSF1 ELISA Kit; IGG1 induction factor ELISA Kit; IL 4 ELISA Kit; IL-4 ELISA Kit; IL4 ELISA Kit; IL4_HUMAN ELISA Kit; Il4e12 ELISA Kit; Interleukin 4 ELISA Kit; Interleukin 4 variant 2 ELISA Kit; Interleukin 4; isoform 1 ELISA Kit; Interleukin-4 ELISA Kit; Lymphocyte stimulatory factor 1 ELISA Kit; MGC79402 ELISA Kit; Pitrakinra ELISA Kit |
| Abbreviation | IL4 |
| Uniprot No. | P05112 |
| Species | Homo sapiens (Human) |
| Sample Types | serum, plasma, tissue homogenates. |
| Detection Range | 31.25 pg/ml - 2000 pg/ml |
| Sensitivity | 7.8 pg/ml |
| Assay Time | 1-5h |
| Sample Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology
|
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Materials provided | - A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human IL-4 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled IL-4 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
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| Materials not provided | - A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator that can provide stable incubation conditions up to 37°C±5°C.
- Squirt bottle, manifold dispenser, or automated microplate washer.
- Absorbent paper for blotting the microtiter plate.
- 100ml and 500ml graduated cylinders.
- Deionized or distilled water.
- Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL and pipette tips.
- Timer
- Test tubes for dilution.
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| ELISA Data Analysis | Watch ELISA data processing video & download Curve Expert if needed |
Troubleshooting
and FAQs | ELISA kit FAQs |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Lead Time | 3-5 working days |
