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홈 > 제품정보 > 시약 > 해외 공식 대리점 > [CSB-E04628r] Rat Interleukin 2,IL-2 ELISA kit
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[CSB-E04628r] Rat Interleukin 2,IL-2 ELISA kit

제품코드 : CSB-E04628r

제품정보 : Rat Interleukin 2,IL-2

  • Sample Types: serum, urine, tissue homogenates
  • Detect Range: 6.25 pg/mL-400 pg/mL
  • Sensitivity: 1.56 pg/mL

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  • 전화번호
    2292-8870

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Product Details

Description
The rat IL-2 ELISA kit is a solid-phase immunoassay specially designed to quantitatively measure rat IL-2 in serum, plasma, or tissue homogenates. It is based on the Sandwich-ELISA mechanism. IL-2 in the sample is bound to the capture antibody immobilized on the 96-well strip plate and then sandwiched with the biotinylated IL-2 antibody. After the addition of HRP-avidin and TMB substrate, the solution in the wells turns blue. The color reaction is stopped by adding the stop solution into the wells, and the color changes from blue to yellow. The color intensity is positively proportional to the...Read more
Target Nameinterleukin 2
Alternative NamesIl2 ELISA kit; Il-2Interleukin-2 ELISA kit; IL-2 ELISA kit; T-cell growth factor ELISA kit; TCGF ELISA kit
AbbreviationIL2
Uniprot No.P17108
SpeciesRattus norvegicus (Rat)
Sample Typesserum, urine, tissue homogenates
Detection Range6.25 pg/mL-400 pg/mL
Sensitivity1.56 pg/mL
Assay Time1-5h
Sample Volume50-100ul
Detection Wavelength450 nm
Research AreaImmunology
Assay Principlequantitative
MeasurementSandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%   
Three samples of known concentration were tested twenty times on one plate to assess. 
Inter-assay Precision (Precision between assays): CV%<10%   
Three samples of known concentration were tested in twenty assays to assess.  
       
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　SampleSerum(n=4) 
1:1Average %92 
Range %87-98 
1:2Average %98 
Range %91-104 
1:4Average %89 
Range %84-99 
1:8Average %96 
Range %92-100 
Recovery
The recovery of rat IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange 
Serum (n=5)9085-96 
       
       
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected 
4002.4892.4782.4842.289 
2001.9541.8961.9251.730 
1001.2921.2731.2831.088 
500.7970.7680.7830.588 
250.5030.4890.4960.301 
12.50.3640.3560.3600.165 
6.250.2760.2580.2670.072 
00.1980.1920.195　 
ELISA Data AnalysisWatch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs		ELISA kit FAQs
StorageStore at 2-8°C. Please refer to protocol.
Lead Time3-5 working days

Citations

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