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> 제품정보 > 시약 > 해외 공식 대리점 > [CSB-E08054m] Mouse Interleukin 1β,IL-1β ELISA Kit
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[CSB-E08054m] Mouse Interleukin 1β,IL-1β ELISA Kit

제품코드 : CSB-E08054m

제품정보 : Mouse Interleukin 1β,IL-1β

  • Sample Types: serum, plasma, cell culture supernates, tissue homogenates
  • Detect Range: 31.25 pg/ml-2000 pg/ml
  • Sensitivity: 7.8 pg/ml

제품문의

  • 전화번호
    2292-8870

제품상세

Product Details

Description

This Mouse IL1B ELISA Kit was designed for the quantitative measurement of Mouse IL1B protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.

Alternative NamesIl1bInterleukin-1 beta ELISA Kit; IL-1 beta ELISA Kit
AbbreviationIL1B
Uniprot No.P10749
SpeciesMus musculus (Mouse)
Sample Typesserum, plasma, cell culture supernates, tissue homogenates.
Detection Range31.25 pg/ml-2000 pg/ml.
Sensitivity7.8 pg/ml.
Assay Time1-5h
Sample Volume50-100ul
Detection Wavelength450 nm
Research AreaImmunology
Assay Principlequantitative
MeasurementSandwich
Materials provided
    • A micro ELISA plate --- The 96-well plate has been pre-coated with anti-mouse IL-1β This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • Biotin-labeled IL-1β antibody (100 x concentrate) 1 x 120 μl ---Act as the detection antibody.
    • HRP-avidin (100 x concentrate) 1 x 120 μl --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • Biotin-antibody Diluent 1 x 15 ml ---Dilute the high concentration Biotin-antibody to an appropriate working solution.
    • HRP-avidin Diluent 1 x 15 ml ---Dilute the high concentration HRP-avidin solution to an appropriate solution.
    • Sample Diluent 1 x 50 ml---Dilute the sample to an appropriate concentration.
    • Wash Buffer (25 x concentrate) 1 x 20 ml --- Wash away unbound or free substances.
    • TMB Substrate 1 x 10 ml --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • Stop Solution 1 x 10 ml --- Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
    • An instruction manual
Materials not provided
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution
ELISA Data AnalysisWatch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
StorageStore at 2-8°C. Please refer to protocol.
Lead Time3-5 working days

Citations