Description | This Mouse IL1B ELISA Kit was designed for the quantitative measurement of Mouse IL1B protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL. |
Alternative Names | Il1bInterleukin-1 beta ELISA Kit; IL-1 beta ELISA Kit |
Abbreviation | IL1B |
Uniprot No. | P10749 |
Species | Mus musculus (Mouse) |
Sample Types | serum, plasma, cell culture supernates, tissue homogenates. |
Detection Range | 31.25 pg/ml-2000 pg/ml. |
Sensitivity | 7.8 pg/ml. |
Assay Time | 1-5h |
Sample Volume | 50-100ul |
Detection Wavelength | 450 nm |
Research Area | Immunology
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Assay Principle | quantitative |
Measurement | Sandwich |
Materials provided | - A micro ELISA plate --- The 96-well plate has been pre-coated with anti-mouse IL-1β This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- Biotin-labeled IL-1β antibody (100 x concentrate) 1 x 120 μl ---Act as the detection antibody.
- HRP-avidin (100 x concentrate) 1 x 120 μl --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- Biotin-antibody Diluent 1 x 15 ml ---Dilute the high concentration Biotin-antibody to an appropriate working solution.
- HRP-avidin Diluent 1 x 15 ml ---Dilute the high concentration HRP-avidin solution to an appropriate solution.
- Sample Diluent 1 x 50 ml---Dilute the sample to an appropriate concentration.
- Wash Buffer (25 x concentrate) 1 x 20 ml --- Wash away unbound or free substances.
- TMB Substrate 1 x 10 ml --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- Stop Solution 1 x 10 ml --- Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
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Materials not provided | - A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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ELISA Data Analysis | Watch ELISA data processing video & download Curve Expert if needed |
Troubleshooting and FAQs | ELISA kit FAQs |
Storage | Store at 2-8°C. Please refer to protocol. |
Lead Time | 3-5 working days |