| Description | The Rat follicle-stimulating hormone (FSH) ELISA Kit is designed for the quantitative determination of endogenic rat FSH concentrations in serum, plasma, or tissue homogenates. This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FSH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FSH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FSH is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is ad...Read more |
| Alternative Names | FshbFollitropin subunit beta ELISA kit; Follicle-stimulating hormone beta subunit ELISA kit; FSH-B ELISA kit; FSH-beta ELISA kit; Follitropin beta chain ELISA kit |
| Abbreviation | FSH |
| Uniprot No. | P18427 |
| Species | Rattus norvegicus (Rat) |
| Sample Types | |
| Detection Range | |
| Sensitivity | |
| Assay Time | 1-5h |
| Sample Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Signal Transduction
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| Assay Principle | quantitative |
| Measurement | Competitive |
| Materials provided | - A 96-well Assay plate -- The 96-well plate has been pre-coated with goat-anti-rabbit FSH antibody.
- Standard 6 x 0.5 ml -- Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
- Anti-rat FSH Antibody 1 x 6 ml -- Act as the detection antibody.
- HRP-conjugated FSH 1 x 6 ml -- Bind to the FSH antibody, and HRP catalyzes the substrate A and B to elicit a chromogenic reaction.
- Wash Buffer (20 x concentrate) 1 x 15 ml -- Wash away unbound or free substances.
- Substrate A 1 x 7 ml -- Mix with substrate B, and the TMB is catalyzed by HRP to develop color.
- Substrate B 1 x 7 ml -- Mix with substrate A, and the TMB is catalyzed by HRP to develop color.
- Stop Solution 1 x 7 ml -- Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells)
- An Instruction manual
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| Materials not provided | - A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
- An incubator that can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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| ELISA Data Analysis | Watch ELISA data processing video & download Curve Expert if needed |
Troubleshooting
and FAQs | ELISA kit FAQs |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Lead Time | 3-5 working days |
