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홈 > 제품정보 > 시약 > 해외 공식 대리점 > [1426-050] Human Transforming Growth Factor-beta 1, accession # PO1137, Ala279-Ser390

[1426-050] Human Transforming Growth Factor-beta 1, accession # PO1137, Ala279-Ser390

제품코드 : 1426-050

제품정보 : CellGenix® Recombinant Human TGF-β1 reliably promotes the expansion of induced pluripotent stem cells (iPSC), embryonic stem cells (ESC), mesenchymal stem cells (MSC) as well as regulatory T cells (Tregs). It also stimulates the differentiation of T cells

Native folding & glycosylation: expressed in human CAP® cells
Safe and ADCF: CAP® cell line extensively characterized and tested for viruses
Global compliance: USP <1043>, Ph. Eur. 5.2.12, ISO TS 20399

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전화번호

2292-8870

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dreamcell@dreamcell.co.kr

제품상세

General Information

Transforming Growth Factor-beta 1 (TGF-β1) is a member of the growth factor beta superfamily and plays a role in various cellular functions like cell differentiation, proliferation and apoptosis. It is secreted by most immune cells and especially interacts with T cells, B cells, macrophages and monocytes.

TGF-β1 is used in the cell and gene therapy space to promote expansion of iPSC, ESC, MSC and Tregs.

CellGenix® Recombinant Human TGF-β1 is produced using a human amniocyte cell line (CAP®). By using the CAP® technology we are able to produce this difficult to express glyco-protein with authentic human post-translational modifications. These human post-translational modifications are not accessible by bacterial expression.

Technical Details

Source

Expressed in CAP® cell line

Description

Human Transforming Growth Factor-beta 1, accession # PO1137, Ala279-Ser390

Formulation

Lyophilized from a 0.2 µm-filtered solution containing 1% Mannitol

The production cell line was derived from an extensively characterized human amniocyte cell line (CAP®).
CAP® is a registered trademark of CEVEC Pharmaceuticals GmbH, Germany.

	Preclinical	GMP
Starting Material	CAP® MCB characterized according to ICH Guidelines Q5A and Q5D Biologics Master File (BB-MF) for the originating CAP® cell bank available	CAP® MCB characterized according to ICH Guidelines Q5A and Q5D Biologics Master File (BB-MF) for the originating CAP® cell bank available
Molecular weight	25.6 kDa per homodimer	25.6 kDa per homodimer
Purity	≥ 95% as determined by SDS-PAGE	≥ 97% as determined by SDS-PAGE
Activity	≥ 9 x 10⁶ IU/mg, calibrated against NIBSC #89/514 Measured in a cell proliferation assay using a TGF-β1-dependent cell line, HT2 clone A5E	≥ 9 – 36 x 10⁶ IU/mg, calibrated against NIBSC #89/514 Measured in a cell proliferation assay using a TGF-β1-dependent cell line, HT2 clone A5E Batch specific activity on CoA
Endotoxin level	≤ 10 EU/mg	≤ 10 EU/mg
Intended use	Intended for preclinical ex vivo use. Not intended for therapeutic use.	For further manufacturing use.

Handling Instructions

Reconstitution

Recommended in sterile water to a final concentration of 250 µg/ml.

Shipment

Ambient temperature. Please refer to our Technote Shipment of CellGenix® Preclinical and GMP Cytokines at Ambient Temperatures.

Expiry

Please refer to Certificate of Analysis for the exact expiry date.

Storage & Stability

Store lyophilized cytokine at -20°C to -80°C.

Avoid repeated freeze/thaw cycles.