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홈 > 제품정보 > 장비 > 유세포 분석기 (Beckman) > Analyzer > CytoFLEX nano 유세포 분석기
CytoFLEX nano 유세포 분석기
제품코드 :
제품정보 : Explore the full picture of your EV experiment with the CytoFLEX nano Flow Cytometer. This purpose-built flow cytometer provides greater sensitivity, consistent instrument performance, and flexibility to study your sample.
- Specifically designed for the analysis of nanoparticles, the CytoFLEX nano Flow Cytometer overcomes the limitations of traditional flow cytometry, giving researchers a sensitive, consistent and flexible solution that can help them advance their research.
- The CytoFLEX nano Flow Cytometer is a pivotal development in flow cytometry that allows users to analyze extracellular vesicles (EVs) at least as small as 40 nm* with ease while simultaneously offering up to 6 separate fluorescent channels of detection and 5 side scatter channels, to deliver full characterization and open additional avenues for your nanoparticle research.
제품문의
-
- 전화번호
- 02-2138-7811
- 이메일
- duuree@dreamcell.co.kr
제품상세
CytoFLEX nano Flow Cytometer
Meet the CytoFLEX nano Flow Cytometer. A pivotal development in flow cytometry, the CytoFLEX nano analyzer enables the analysis of EVs at least as small as 40 nm* with ease while simultaneously offering up to 6 separate fluorescent channels of detection, to deliver full characterization.
With the CytoFLEX nano Flow Cytometer, you can expect:
- Increased size sensitivity from 1 μm to at least 40 nm*
- Flexibility for your experimental design with 6 fluorescent channels and 5 side scatter channels
- Consistency of results with >90% volumetric counting accuracy and <1% carryover between samples
*Characterized using 40 nm polystyrene beads and triggering on violet side scatter.
Nanoscale Flow Cytometry
Figure 1: The size of different cells and extracellular vesicles. The CytoFLEX nano Flow Cytometer can detect, characterize, and determine the size of EVs down to at least 40 nm*.
Enhanced Efficiency with More Powerful Optics and Fluidics
The marriage between fluidics and optics is critical when enabling the detection of smaller biological nanoparticles. Based on our CytoFLEX principles, we have fully redesigned optics and fluidics to develop the unique capabilities of the CytoFLEX nano Flow Cytometer:
- Increased laser power and purpose-designed optical excitation path for maximized examination of each particle.
- Sample and sheath flow rates are reduced* to increase exposure times, helping to collect more light and detect the EVs in your sample with more efficiency.
- Sample delivery via piston pump provides more stability and accuracy of volumetric counting.
- Sheath fluid paired with a user-exchangeable 5 nm filter and baseline monitoring options minimizes noise from debris.
- Daily QC check-ups eliminate nano and micro bubble elimination and control debris.
*Compared to CytoFLEX flow cytometer
OPTICS
EXCITATION OPTICS
The CytoFLEX nano flow cytometer has the capacity for 12 simultaneous detection channels, broken out as 6 fluorescent channels, 5 side scatter channels and 1 forward scatter channel. Each instrument is available in one configuration with all lasers activated. The excitation lasers are listed in the table below.
LASER SPECIFICATIONS
Colinear Laser Description
| Laser | Wavelength | Power at Flow Cell |
| Violet | 405 nm | 120 mW |
| Blue | 488 nm | 50 mW |
| Yellow | 561 nm | 35 mW |
| Red | 638 nm | 100 mW |
PERFORMANCE
| CytoFLEX nano Flow Cytometer Performance | ||
| Violet side scatter sensitivity | VSSC1: 40 nm relative to polystyrene nanoparticles VSSC2: 80 nm relative to polystyrene nanoparticles | |
| Scatter detection dynamic range | VSSC 1 for small range 40-150 nm polystyrene nanoparticles VSSC2 for large range 80-1000 nm polystyrene nanoparticles | |
| Violet forward scatter sensitivity | 300 nm relative to polystyrene nanoparticles | |
| Fluorescence sensitivity and resolution | Simultaneous detection of fluorescence on six fluorescence detectors, using 500 nm CytoFLEX nano Multi-intensity Fluorospheres V477: 8 peaks B531: 8 peaks Y595: 8 peaks R670: 6 peaks R710: 5 peaks R792: 4 peaks | |
| Fluorescence rCV | CV < 10% (using QC Fluorospheres at 1 µL/min) | |
| Carryover between Samples | ≤1%a | |
| Acquisition speed | Maximum electronic acquisition speed | 16000 events/second with ≤ 95% yield |
| Recommended maximum sample acquisition speed | 5000 events/second to avoid possible swarming or coincidence situations. | |
| Volumetric Counting accuracy | > 90%b | |
a. Tested with polystyrene nanoparticles.
b. Tested with 144 nm QC Scatterspheres on 3 units under the conditions: record 3 minutes at the sample flow rates of 1μL/min, 2μL/min, 3μL/min,
4μL/min, 5μL/min, 6μL/min respectively, repeat 5 times, then calculated the average total events for each speed, compared the calculated the
volumetric counting accuracy with the theoretical total events (the known concentration 1.3*108 x volume).
How to Use the CytoFLEX nano Flow Cytometer
