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> 제품정보 > 시약 > 해외 공식 대리점 > Recombinant Human Neurofilament light polypeptide(NEFL)
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Recombinant Human Neurofilament light polypeptide(NEFL)

제품코드 : CSB-EP015688HU

제품정보 : Others

  • Size: 1mg/100ug/20ug
  • Express system: E.coli
  • Species: Homo sapiens (Human)
  • Tag Info: N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged

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  • 전화번호
    2292-8870

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Product Details

Description
The recombinant human neurofilament light polypeptide (NEFL) is prepared in the E.coli. Its expression region lies in the fragment encoding amino acid residues 2-543 of the human NEFL protein. This recombinant human NEFL protein is labeled with a 10xHis-SUMO-tag at the N-terminus and a Myc-tag at the C-terminus. It harbors the full length of the mature protein in length. The purity of this NEFL protein is greater than 90% determined by SDS-PAGE. On the gel, this protein migrated to the band with a molecular weight of about 90 kDa. And its component has been identified by the LC-MS/MS Analysis....Read more
PurityGreater than 90% as determined by SDS-PAGE.
Target NamesNEFL
Uniprot No.P07196
Research Areaothers
Alternative NamesNEFL; NF68; NFL; Neurofilament light polypeptide; NF-L; 68 kDa neurofilament protein; Neurofilament triplet L protein
SpeciesHomo sapiens (Human)
SourceE.coli
Expression Region2-543aa
Target Protein SequenceSSFSYEPYYSTSYKRRYVETPRVHISSVRSGYSTARSAYSSYSAPVSSSLSVRRSYSSSSGSLMPSLENLDLSQVAAISNDLKSIRTQEKAQLQDLNDRFASFIERVHELEQQNKVLEAELLVLRQKHSEPSRFRALYEQEIRDLRLAAEDATNEKQALQGEREGLEETLRNLQARYEEEVLSREDAEGRLMEARKGADEAALARAELEKRIDSLMDEISFLKKVHEEEIAELQAQIQYAQISVEMDVTKPDLSAALKDIRAQYEKLAAKNMQNAEEWFKSRFTVLTESAAKNTDAVRAAKDEVSESRRLLKAKTLEIEACRGMNEALEKQLQELEDKQNADISAMQDTINKLENELRTTKSEMARYLKEYQDLLNVKMALDIEIAAYRKLLEGEETRLSFTSVGSITSGYSQSSQVFGRSAYGGLQTSSYLMSTRSFPSYYTSHVQEEQIEVEETIEAAKAEEAKDEPPSEGEAEEEEKDKEEAEEEEAAEEEEAAKEESEEAKEEEEGGEGEEGEETKEAEEEEKKVEGAGEEQAAKKKD
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight81.4kDa
Protein LengthFull Length of Mature Protein
Tag InfoN-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged
FormLiquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
ReconstitutionWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage ConditionStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf LifeThe shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time3-7 business days
NotesRepeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COAPlease contact us to get it.

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