인류의 건강과 생명공학 발전을 위해 봉사하는 기업 드림셀
We Serving the Health and Biotechnology of Humanity
홈 > 제품정보 > 시약 > 국내 공식 대리점 > Lumit™ HMGB1 (Human/Mouse) Immunoassay, 100 assays
Lumit™ HMGB1 (Human/Mouse) Immunoassay, 100 assays
제품코드 : W6110
제품정보 : Inflammation Assay
- Lumit Immunoassay는 washing, transfer, sample dilution 단계없이 세포 배양 상태에서 바로 analyte를 특정할 수 있는 새로운 immunoassay입니다.
- [W6110] 100 assays, [W6112] 500 assays
제품문의
-
- 전화번호
- 2292-8870
- 이메일
- dreamcell@dreamcell.co.kr
제품상세
Fast, No-Wash Detection of HMGB1
High-Mobility Group Box 1 (HMGB1) functions as a damage-associated molecular pattern (DAMP) released from cells in inflammatory responses. HMGB1 is often measured as a key biomarker of immunogenic cell death, a specialized form of cell death that results in an immune response. HMGB1 also functions as a proinflammatory cytokine. Current methods to detect HMGB1, such as ELISA, require tedious wash steps and lengthy protocols. Lumit™ technology simplifies HMGB1 quantitation with an add-mix-measure protocol completed in 90 minutes or less.
How the Lumit™ HMGB1 Immunoassay Works
Primary antibodies to HMGB1, selected for their specific and sensitive detection, are labeled with the LgBiT and SmBiT subunits of NanoBiT® Luciferase. In the presence of HMGB1, the subunits are brought together to form an active luciferase enzyme. Addition of optimized substrate generates a bright luminescence signal proportional to HMGB1 levels. The assay detects both human and mouse HMGB1.
Simple One-Plate Protocol, No Wash Steps
Large Dynamic Range of HMGB1 Detection
Due to the homology of human and mouse HMGB1, the kit can be used for both species with minimal performance differences.
Target | Human HMGB1 | Mouse HMGB1 |
Limit of Detection | 1ng/ml | 3ng/ml |
Dynamic Range | 4–1,000ng/ml | 3–2,187ng/ml |
Assay Time | 90 minutes | |
Sample Type | Cell culture samples | |
SD=Standard Deviation
Dual DAMP Measurements in a Human Cell Line
In parallel assay plates, U937 cells were dosed with mitoxantrone and assayed for extracellular ATP release (left) kinetically over 24 hours using the RealTime-Glo™ Extracellular ATP Assay. Lumit™ HMGB1 Immunoassay reagents were added directly to the second plate of treated cells after 24 hours of treatment. Mitoxantrone produces a dose- and time-dependent release of extracellular ATP (left) and a dose-dependent release of HMGB1 (right) with potency agreement between the two measurements of damage-associated molecular patterns.
Detect Drug-Induced Immunogenic Cell Death in Various Cell Culture Models
Human U2OS cells (left) and mouse EL4 cells (right) were treated with doxorubicin for 24 hours. After 24 hours, the Lumit™ HMGB1 Immunoassay was performed directly on cell culture samples to measure HMGB1 released from treated cells.