제품상세

Mismatiching을 수정해 주는 proofreading 기능이 포함된 제품입니다. 

High Fidelity DNA Synthesis

• Lowest error rate of any thermostable DNA polymerase
• Provided with 10X buffer containing 20mM MgSO₄
• Recommended for applications that require high accuracy base insertion

Pfu DNA Polymerase is a high-fidelity, thermostable enzyme of approximately 90kDa isolated from Pyrococcus furiosus. The enzyme replicates DNA at 75°C, catalyzing the polymerization of nucleotides in the 5´→3´ direction in the presence of magnesium. Pfu DNA Polymerase also possesses 3´→5´ exonuclease (proofreading) activity. Base misincorporations that may occur during polymerization are rapidly excised by this proofreading activity. Consequently, Pfu DNA Polymerase is recommended for use in PCR and primer extension reactions that require high-fidelity synthesis. Pfu DNA Polymerase-generated PCR fragments are blunt-ended.

Performance Guarantee: Promega PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any Promega PCR product, we will send a replacement or refund your account.

1. Fiala, G. and Stetter, K.O. (1986) Arch. Microbiol. 145, 56.
2. Lundberg, K.S. et al. (1991) Gene 108, 1–6.
3. Flaman, J.M. et al. (1994) Nucl. Acids Res. 22, 3259–60.
4. Cline, J. et al. (1996) Nucl. Acids Res. 24, 3546–51.
5. Andre, P. et al. (1997) Genome Res. 7, 843–52.

 

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Pfu DNA Polymerase Product Information 9PIM7741