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Room-Temperature PCR Setup in a Ready-to-Use Master Mix Format
- Eliminate nonspecific amplification with a hot start enzyme
- All-in-one master mix: just add template and primers
- Choose formulations with or without loading dye
Convenient, Room-Temperature Setup and Higher Performance GoTaq® G2 Enzyme
GoTaq® G2 Hot Start Polymerase is bound to a proprietary antibody that blocks activity at temperatures below 70°C for convenient, room-temperature reaction setup. Polymerase activity is restored during the initial denaturation step, when amplification reactions are heated at 94–95°C for two minutes, allowing hot-start PCR following room-temperature reaction assembly. Hot-start PCR is advantageous for some amplification targets because it may eliminate or minimize primer-dimer and nonspecific products. In some cases, hot-start PCR may improve yields. GoTaq® G2 Hot Start Polymerase also exhibits 5´→3´ exonuclease activity.
GoTaq® G2 Hot Start Master Mix displays superior sensitivity and robust yield.
GoTaq® G2 Hot Start Master Mixes are ready-to-use mixes containing all necessary components (GoTaq® G2 Hot Start Polymerase, buffer, dNTPs and optimized magnesium)—you only need to add primer and template, and go!
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