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홈 > 제품정보 > 시약 > 국내 공식 대리점 > GoTaq® 2-Step RT-qPCR System, 5ml
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GoTaq® 2-Step RT-qPCR System, 5ml

제품코드 : A6010

제품정보 : Real-time PCR

  • Dye-based (BRYT Green® Dye) quantitative PCR assays 제품입니다.
  • 제품내 포함 된 시약을 이용해 RNA의 발현 및 정량을 다음의 두 과정으로 진행합니다
  • 1. GoScript™ Reverse Transcription System으로 first-strand cDNA 합성
  • 2. 합성된 cDNA와 GoTaq® qPCR Master Mix를 이용해 실시간 정량분석

제품문의

  • 전화번호
    2292-8870

제품상세

Convenient Master Mixes for Real-Time  RT-qPCR

  • Ready-to-use mix of optimized components for dye-based  RT-qPCR
  • Compatible with both fast and standard cycling methods
  • Resistant to a wide range of PCR inhibitors

Simple, Sensitive, Dye-Based qPCR and RT-qPCR

The GoTaq® qPCR and RT-qPCR Systems are ready-to-use, 2X master mixes containing BRYT Green® Dye, a  fluorescent DNA binding dye with minimal PCR inhibition, providing maximum amplification efficiency and greater fluorescence enhancement than SYBR® Green I.

Rapid hot-start activation and processive enzymes make the  GoTaq® Systems compatible with most real-time PCR instruments using standard or fast cycling. The 1-Step and 2-Step RT-qPCR Systems also include GoScript™ Reverse Transcriptase, to enable efficient synthesis of first-strand cDNA in preparation for PCR.



Two-Step Reverse Transcription and qPCR

The GoTaq® 2-Step RT-qPCR System facilitates detection and relative quantification of RNA expression levels via a two-step RT-qPCR method using GoScript™ Reverse Transcription System and GoTaq® qPCR Master Mix.

The GoTaq® qPCR Master Mix is provided as a ready-to-use, stabilized 2X formulation and contains GoTaq® Hot Start Polymerase, MgCl2, dNTPs, BRYT Green® Dye and a proprietary reaction buffer. This master mix contains a low level of carboxy-X-rhodamine (CXR); a separate tube of CXR reference dye is included.

The GoScript™ Reverse Transcription System includes an optimized reaction buffer and M-MLV reverse transcriptase designed to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. The cDNA product can be added directly to downstream qPCR amplification reactions.



Extended linear range. Dilutions of human reference total RNA were amplified with GoTaq® 2-Step RT-qPCR System looking at 5.8S expression. GoTaq® 2-Step system demonstrates a linear dynamic range over 9 orders of  magnitude with a high fluorescent signal. NTC reactions were all blank.

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