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홈 > 제품정보 > 시약 > 국내 공식 대리점 > AccuMAP™ Low pH Protein Digestion Mini Kit, 10 reactions
제품명

AccuMAP™ Low pH Protein Digestion Mini Kit, 10 reactions

제품코드 : VA1040

제품정보 : Protease

  • Protein digestion 과정에서 발생할 수 있는 artificial non-enzymatic PTMs을 억제하여 정확하고 재현성 높은 분석이 가능합니다.
  • [VA1040] Mini Kit 10 rxns
  • [VA1041] Maxi kit 100 rxns

제품문의

  • 전화번호
    2292-8870

제품상세

Suppress Artificial Deamidation and Disulfide Bond Scrambling in Protein Sample Prep

  • Sample preparation completed in 4.5–5 hours
  • Highly reproducible digestion results
  • Use for reduced and nonreduced proteins

Suppress Nonenzymatic PTMs During Protein Sample Prep

Nonenzymatic posttranslational modifications (PTMs) spontaneously occur in biotherapeutic proteins during manufacturing and storage. The major nonenzymatic PTMs are deamidation, disulfide bond scrambling and oxidation. These modifications can affect efficacy and stability of biotherapeutic proteins and are the subject of careful monitoring.

Nonenzymatic PTMs can also be induced during protein sample preparation for peptide mapping and can compromise analysis. Major causes of nonenzymatic PTMs induced during sample preparation include alkaline pH and impurities that have protein oxidizing activity.



Suppress Artificial Nonenzymatic PTMs

The AccuMAP™ Low pH Protein Digestion Kits suppress artificial nonenzymatic PTMs, as sample preparation is performed at low pH under either reducing or nonreducing conditions.

13959MA-W
Schematic diagram of sample preparation by digestion performed with AccuMAP™ Low pH Protein Digestion Kit.

Suppression of Deamidation and Disulfide Bond Scrambling


13962MA-W-a-2
13962MA-W-b-2

Complete suppression of deamidation and disulfide bond scrambling in IgG digested with AccuMAP™ Low pH Protein Digestion Kit. Figure shows extracted ion chromatogram of a GLEWIGAIYPGNGDTSYNQK peptide from Rituximab antibody digested at conventional conditions (pH 8) and at low pH (with AccuMAP™ Low pH Protein Digestion Kit). The data show suppression of deamidation in the upper panel and the complete suppression of disulfide bond scrambling in the lower panel.

Minimal Baseline Noise in Tryptic Digests

13963MA-W-a-2
13963MA-W-b-2

UV-HPLC chromatograms of Panitumumab digests. Panitumumab was predigested with AccuMAP™ Low pH Resistant rLys-C, then the reaction was diluted and digestion was completed by incubation with trypsin for 3 hours (upper panel) or overnight (lower panel). In this experiment we used a 1:5 trypsin:protein ratio. Note the accumulation of baseline noise for the overnight digest shown in the lower panel.




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