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> 제품정보 > 시약 > 국내 공식 대리점 > Trypsin/Lys-C Mix, Mass Spec Grade, 100ug
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Trypsin/Lys-C Mix, Mass Spec Grade, 100ug

제품코드 : V5072

제품정보 : Protease

  • Mass Spec Grade의 Trypsin Gold와 rLsy-C mixture 제품으로 proteolysis 기능이 증가되어 재현성 높은 실험 결과를 제공합니다.
  • [V5071] 20ug, [V5072] 100ug, [V5073] 5 X 20ug

제품문의

  • 전화번호
    2292-8870

제품상세

Improve Protein Digestion with Mix of Trypsin Gold and rLys-C

  • Standard overnight digestion, nondenaturing conditions
  • Increased peptide recovery, enhanced protein quantitation, improved reproducibility
  • Tolerant to trypsin-inhibiting contaminants

Increased Peptide and Protein Identifications Using a Standard Trypsin Digestion Protocol

Standard overnight trypsin digest schematic 11788ME-W-a
Trypsin LysC overnight digest schematic 11788ME-W-b
Data comparing trypsin and Trypsin/Lys-C digests.
Incomplete digestion with trypsin was evident after comparing peptide peak intensities in trypsin and Trypsin/Lys-C Mix digests.

Improved Reproducibility using Trypsin/Lys-C

Reproducible IgG (Rituximab) digestion  with Trypsin Lys-C mix.
Improved reproducibility of IgG (Rituximab) digestion when using the Trypsin/Lys-C Mix.


Tolerant To Trypsin-Inhibiting Contaminants

Trypsin LysC digestion results in fewer missed cleavages. 13058MC-W-a
Trypsin LysC increases protein identification compared to trypsin alone. 13058MC-W-b
Efficient digestion with Trypsin/Lys-C Mix in the presence of trypsin-inhibiting agents. Yeast protein extract was used as a model system.
Conclusions: Use of Trypsin/Lys-C resulted in fewer missed cleavages and a 20% increase in protein identifications.


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Trypsin/Lys-C Mix, Mass Spec Grade Technical Manual TM390