제품상세

Maximum Trypsin Digest Specificity, Extreme Resistance to Autolytic Digestion

• Each lot qualified by mass spectrometry to ensures compatibility with your applications/instrumentation
• Chymotrypsin activity eliminated for distinct, consistent data
• Digest in gel, or in solution

Excellent Specificity and Resistance to Autolytic Digestion

Trypsin Gold, Mass Spectrometry Grade, is manufactured to provide maximum specificity. Lysine residues in the porcine trypsin have been modified by reductive methylation, yielding a highly active and stable molecule that is extremely resistant to autolytic digestion. The specificity of the purified trypsin is further improved by TPCK treatment, which inactivates chymotrypsin. The treated trypsin is then purified by affinity chromatography and lyophilized to yield Trypsin Gold, Mass Spectrometry Grade.

Spectrogram Showing Digestion with Trypsin Gold, Mass Spec Grade

A 500ng sample of carbonic anhydrase II was separated by gel electrophoresis and digested with 500ng of Trypsin Gold, Mass Spectrometry Grade, overnight at 37°C.

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Trypsin Gold, Mass Spectrometry Grade Technical Bulletin #TB309