How the Assay Works

The Glucose-Glo™ Assay couples glucose oxidation and NADH production with a bioluminescent NADH detection system. Glucose dehydrogenase uses glucose and NAD+ to produce NADH. In the presence of NADH a pro-luciferin Reductase Substrate is converted by Reductase to luciferin that is then used by Ultra-Glo™ Recombinant Luciferase to produce light.

When Glucose Detection Reagent, which contains glucose dehydrogenase (GDH), NAD+, Reductase, Reductase Substrate and Luciferase, is added to a sample containing glucose at a 1:1 ratio, the enzyme-coupled reactions start and run simultaneously. The luminescent signal is proportional to the amount of glucose in the sample and increases until all glucose is consumed, at which time a stable luminescent signal is achieved.

Amenable to Your Throughput and Workflow Requirements

The Glucose-Glo™ Assay is a versatile detection kit that is amenable to high-throughput formats and compatible with many sample types including cells, lysates, tissue, plasma and serum. To simplify sample processing, the glucose detection kit uses processing methods that do not require sample centrifugation or spin columns. Simply dilute your samples, add inactivation and neutralization solutions (if needed), and assay all samples directly in 96- or 384-well plates.