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> 제품정보 > 시약 > 국내 공식 대리점 > Lumit™ Mouse IL-1β Immunoassay, 100 assays
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Lumit™ Mouse IL-1β Immunoassay, 100 assays

제품코드 : W7010

제품정보 : Inflammation Assay

  • Lumit Immunoassay는 washing, transfer, sample dilution 단계없이 세포 배양 상태에서 바로 analyte를 특정할 수 있는 새로운 immunoassay입니다.
  • [W7010] 100 assays, [W7012] 500 assays, [W7011] 1,000 assays

제품문의

  • 전화번호
    2292-8870

제품상세

No-Wash Immunoassay for IL-1β Detection in Cells

  • Fast add-and-read protocol performed directly on cells
  • Luminescent detection with just a standard plate-reading luminometer
  • Scalable to 96- or 384-well plates

Quantify Inflammasome Activation Without Tedious Wash Steps

The Lumit™ IL-1β Immunoassay quantitatively measures released IL-1β in cell culture samples using a simple, no-wash protocol. Just add labeled antibodies to the sample, add detection reagent and read luminescent signal using a standard plate-reading luminometer. The entire protocol is completed in less than 70 minutes! The assay can be used directly on cells in culture or culture medium transferred to a separate assay plate.

How the Lumit™ IL-1β Immunoassay Works

Primary antibodies to IL-1β were selected for their specific and sensitive detection and labeled with the LgBiT and SmBiT subunits of NanoBiT® Luciferase. In the presence of IL-1β, the subunits are brought together to form an active luciferase enzyme. Addition of optimized substrate generates a bright luminescent signal proportional to IL-1β levels.
16522ma

Simple One-Plate Protocol, No Wash Steps!

16545ma

Large Dynamic Range of Lumit™ Detection

The picomolar to nanomolar linear range of Lumit™ detection covers expected concentrations for most cell models.

Linear range of lumit IL-1b human immunoassay
Linear range of lumit IL-1b mouse immunoassay

Assay

IL-1β (Human) Immunoassay

IL-1β (Mouse) Immunoassay

Limit of Detection (LOD)

10pg/ml (3 SD above background)

8pg/ml (3 SD above background)

Dynamic Range

22pg/ml – 40ng/ml

11pg/ml – 20ng/ml

Minimal Detectable Dose (MDD)

7pg/ml (2 SD above background)

5pg/ml (2 SD above background)

Assay Time

70 minutes

Sample Type

Cell culture supernatants

SD=Standard Deviation

High-Throughput NLRP3 Inflammasome Inhibitor Screening

The add-and-read format of the Lumit™ IL-1β Immunoassay enables high-throughput screening for modulators of IL-1β release.
IL-1b inhibitor high throughput screening

The NLRP3 inflammasome inhibitor, MCC950, prevents caspase-1 activation and release of mature IL-1β. A titration of MCC950 was added to differentiated THP-1 cells (5 × 104/well) followed by LPS (20 EU/ml) or vehicle. The Lumit™ IL-1β (Human) Immunoassay was performed after 5 hours.













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