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홈 > 제품정보 > 시약 > 국내 공식 대리점 > Lumit™ IL-4 (Human) Immunoassay, 100 assays
Lumit™ IL-4 (Human) Immunoassay, 100 assays
제품코드 : W6060
제품정보 : Inflammation Assay
- Lumit Immunoassay는 washing, transfer, sample dilution 단계없이 세포 배양 상태에서 바로 analyte를 특정할 수 있는 새로운 immunoassay입니다.
- [W6060] 100 assays, [W6062] 500 assays, [W6061] 1,000 assays
제품문의
-
- 전화번호
- 2292-8870
- 이메일
- dreamcell@dreamcell.co.kr
제품상세
No-Wash Immunoassay for IL-4 Detection
- Fast add-and-read protocol performed directly on cells
- Luminescent detection with just a plate-reading luminometer
- Scalable to 96- or 384-well plates
Quantify Cytokine Release Without Tedious Wash Steps
The Lumit™ IL-4 Immunoassay quantitatively measures released IL-4 in cell culture samples using a simple, no-wash protocol. Just add labeled antibodies to the sample, add detection reagent and read luminescent signal using a standard plate-reading luminometer. The entire protocol is completed in less than 70 minutes! The assay can be used directly on cells in culture or culture medium transferred to a separate assay plate.
How the Lumit™ IL-4 Immunoassay Works
Primary antibodies to IL-4 were selected for their specific and sensitive detection and labeled with the LgBiT and SmBiT subunits of NanoBiT® Luciferase. In the presence of IL-4, the subunits are brought together to form an active luciferase enzyme. Addition of optimized substrate generates a bright luminescent signal proportional to IL-4 levels.
Simple One-Plate Protocol, No Wash Steps
Large Dynamic Range of Lumit™ IL-4 Detection
The pg/ml to ng/ml linear range of Lumit™ detection covers expected concentrations for most cell models.
Assay | IL-4 Human Immunoassay | |
Limit of Detection (LOD) | 6.7pg/ml (3 SD above background) | |
Dynamic Range | 18.2pg/ml – 25ng/ml | |
Minimal Detectable Dose (MDD) | 4.5pg/ml (2 SD above background) | |
Assay Time | 70 minutes | |
Sample Type | Cell culture supernatants | |
SD=Standard Deviation
Sensitive Quantitation of Low-Level IL-4 Release
When treated with a Cell Stimulation Cocktail (CSC), T helper type 2 (Th2) cells and human PBMCs (PBMC) each release low levels of IL-4. Following 24 hours of CSC treatment, Lumit™ IL-4 Immunoassay reagents were added directly to cell wells to measure IL-4 release.
Same-Well Multiplexing with Viability and Cytotoxicity Assays
Human T helper type 2 (Th2) cells were plated in 96-well plate format, primed overnight with hIL-2 and treated with increasing concentration of cell stimulation cocktail (CSC) for 24 hours. Following treatment, CellTox™ Green Cytotoxicity Assay and the Lumit™ IL-4 (Human) Immunoassay reagents were added to determine cytotoxicity and IL-4 release. CellTiter-Glo® Cell Viability Assay reagent was added to determine cell viability. A dose-dependent increase in IL-4 was observed, with some degree of increased cytotoxicity and decreased cell viability.