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홈 > 제품정보 > 시약 > 국내 공식 대리점 > Lumit™ TNF-α (Human) Immunoassay, 100 assays
Lumit™ TNF-α (Human) Immunoassay, 100 assays
제품코드 : W6050
제품정보 : Inflammation Assay
- Lumit Immunoassay는 washing, transfer, sample dilution 단계없이 세포 배양 상태에서 바로 analyte를 특정할 수 있는 새로운 immunoassay입니다.
- [W6050] 100 assays, [W6052] 500 assays, [W6051] 1,000 assays
제품문의
-
- 전화번호
- 2292-8870
- 이메일
- dreamcell@dreamcell.co.kr
제품상세
No-Wash Immunoassay for TNF-α Detection
- Fast add-and-read protocol performed directly on cells
- Luminescent detection with just a plate-reading luminometer
- Scalable to 96- or 384-well plates
Quantify Cytokine Release Without Tedious Wash Steps
The Lumit™ TNF-α Immunoassay quantitatively measures released TNF-α in cell culture samples using a simple, no-wash protocol. Just add labeled antibodies to the sample, add detection reagent and read luminescent signal using a standard plate-reading luminometer. The entire protocol is completed in less than 70 minutes! The assay can be used directly on cells in culture or culture medium transferred to a separate assay plate.
How the Lumit™ TNF-α Immunoassay Works
Primary antibodies to TNF-α were selected for their specific and sensitive detection and labeled with the LgBiT and SmBiT subunits of NanoBiT® Luciferase. In the presence of TNF-α, the subunits are brought together to form an active luciferase enzyme. Addition of optimized substrate generates a bright luminescent signal proportional to TNF-α levels.
Simple One-Plate Protocol, No Wash Steps
Large Dynamic Range of Lumit™ TNF-α Detection
The pg/ml to ng/ml linear range of Lumit™ detection covers expected concentrations for most cell models.
Assay | TNF-α Human Immunoassay | |
Limit of Detection (LOD) | 2.9pg/ml (3 SD above background) | |
Dynamic Range | 18.2pg/ml – 25ng/ml | |
Minimal Detectable Dose (MDD) | 2.0pg/ml (2 SD above background) | |
Assay Time | 70 minutes | |
Sample Type | Cell culture supernatants | |
SD=Standard Deviation
Sensitive Detection of TNF-α Without Sample Dilution
When treated with a Cell Stimulation Cocktail (CSC), human PBMCs release TNF-α. After treatment for 24, 48 or 72 hours, Lumit™ TNF-α Human Immunoassay reagents were added directly to cell wells to measure TNF-α release. No sample dilution was required to measure quantities of TNF- α release.
Multiple Cytokine Detection by Split-Sample Analysis
Human PBMCs were plated in a 96-well plate in 100µl and treated for 24 hours with cell stimulation cocktail (CSC), lipopolysaccharide (LPS) or R848. Four 20μl aliquots were transferred to a 384-well plate for detection of IL-2, IL-6, IFN-γ and TNF-α with the corresponding Lumit™ Cytokine Immunoassay. This simple approach for profiling cytokine