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홈 > 제품정보 > 시약 > 국내 공식 대리점 > Lumit™ IFN-ɣ (Human) Immunoassay, 100 assays
Lumit™ IFN-ɣ (Human) Immunoassay, 100 assays
제품코드 : W6040
제품정보 : Inflammation Assay
- Lumit Immunoassay는 washing, transfer, sample dilution 단계없이 세포 배양 상태에서 바로 analyte를 특정할 수 있는 새로운 immunoassay입니다.
- [W6040] 100 assays, [W6042] 500 assays, [W6041] 1,000 assays
제품문의
-
- 전화번호
- 2292-8870
- 이메일
- dreamcell@dreamcell.co.kr
제품상세
No-Wash Immunoassay for IFN-γ Detection
- Fast add-and-read protocol performed directly on cells
- Luminescent detection with just a plate-reading luminometer
- Scalable to 96- or 384-well plates
Quantify Cytokine Release Without Tedious Wash Steps
The Lumit™ IFN-γ Immunoassay quantitatively measures released IFN-γ in cell culture samples using a simple, no-wash protocol. Just add labeled antibodies to the sample, add detection reagent and read luminescent signal using a standard plate-reading luminometer. The entire protocol is completed in less than 70 minutes! The assay can be used directly on cells in culture or culture medium transferred to a separate assay plate.
How the Lumit™ IFN-γ Immunoassay Works
Primary antibodies to IFN-γ were selected for their specific and sensitive detection and labeled with the LgBiT and SmBiT subunits of NanoBiT® Luciferase. In the presence of IFN-γ, the subunits are brought together to form an active luciferase enzyme. Addition of optimized substrate generates a bright luminescent signal proportional to IFN-γ levels.
Simple One-Plate Protocol, No Wash Steps
Large Dynamic Range of Lumit™ IFN-γ Detection
The pg/ml to ng/ml linear range of Lumit™ detection covers expected concentrations for most cell models.
Assay | IFN-γ Human Immunoassay | |
Limit of Detection (LOD) | 1.7pg/ml (3 SD above background) | |
Dynamic Range | 7.3pg/ml – 10ng/ml | |
Minimal Detectable Dose (MDD) | 1.1pg/ml (2 SD above background) | |
Assay Time | 70 minutes | |
Sample Type | Cell culture supernatants | |
SD=Standard Deviation
Detect IFN-γ as a Marker of T Cell Activation
Purified CD8+ T cells (effector cells) were combined with Raji B cells (target cells) and a serial dilution of Blincyto® (CD3 and CD19 bispecific T cell engager). The Lumit™ IFN-γ (Human) Immunoassay reagents were added directly to treated cell wells to detect IFN-γ release from the T cells.
Multiple Cytokine Detection by Split-Sample Analysis
Human PBMCs were plated in a 96-well plate in 100µl and treated for 24 hours with cell stimulation cocktail (CSC), lipopolysaccharide (LPS) or R848. Four 20μl aliquots were transferred to a 384-well plate for detection of IL-2, IL-6, IFN-γ and TNF-α with the corresponding Lumit™ Cytokine Immunoassay. This simple approach for profiling cytokine release can be applied to multiple cell models.
Explore Additional Cytokine Detection Assays
We offer a growing portfolio of assays to detect cytokines in cell culture samples.
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For a biotech that understands time is of significant value, the Lumit™ Immunoassays are an essential way for us to gather reliable data fast so that we can reach conclusions and make decisions sooner.
Jonathan Chow, Principal Scientist, Corner Therapeutics