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홈 > 제품정보 > 시약 > 국내 공식 대리점 > Lumit™ IL-2 (Human) Immunoassay, 100 assays
Lumit™ IL-2 (Human) Immunoassay, 100 assays
제품코드 : W6020
제품정보 : Inflammation Assay
- Lumit Immunoassay는 washing, transfer, sample dilution 단계없이 세포 배양 상태에서 바로 analyte를 특정할 수 있는 새로운 immunoassay입니다.
- [W6020] 100 assays, [W6022] 500 assays, [W6021] 1,000 assays
제품문의
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- 전화번호
- 2292-8870
- 이메일
- dreamcell@dreamcell.co.kr
제품상세
No-Wash Immunoassay for IL-2 Detection
- Fast add-and-read protocol performed directly on cells
- Luminescent detection with just a plate-reading luminometer
- Scalable to 96- or 384-well plates
Quantify Cytokine Release Without Tedious Wash Steps
The Lumit™ IL-2 Immunoassay quantitatively measures released IL-2 in cell culture samples using a simple, no-wash protocol. Just add labeled antibodies to the sample, add detection reagent and read luminescent signal using a standard plate-reading luminometer. The entire protocol is completed in less than 70 minutes! The assay can be used directly on cells in culture or culture medium transferred to a separate assay plate.
How the Lumit™ IL-2 Immunoassay Works
Primary antibodies to IL-2 were selected for their specific and sensitive detection and labeled with the LgBiT and SmBiT subunits of NanoBiT® Luciferase. In the presence of IL-2, the subunits are brought together to form an active luciferase enzyme. Addition of optimized substrate generates a bright luminescent signal proportional to IL-2 levels.
Simple One-Plate Protocol, No Wash Steps
Large Dynamic Range of Lumit™ IL-2 Detection
The pg/ml to ng/ml linear range of Lumit™ detection covers expected concentrations for most cell models.
Assay | IL-2 Human Immunoassay | |
Limit of Detection (LOD) | 11.2pg/ml (3 SD above background) | |
Dynamic Range | 28.2pg/ml – 25ng/ml | |
Minimal Detectable Dose (MDD) | 7.2pg/ml (2 SD above background) | |
Assay Time | 70 minutes | |
Sample Type | Cell culture supernatants | |
SD=Standard Deviation
Detect IL-2 Release as a Marker of T Cell Activation
Purified CD8+ T cells (effector cells) were combined with Raji B cells (target cells) and a serial dilution of Blincyto® (CD3 and CD19 bispecific T cell engager). The Lumit™ IL-2 (Human) Immunoassay reagents were added directly to treated cell wells to detect IL-2 release from the T cells.
Lumit™ IL-2 Immunoassay is Scalable for High-Throughput Applications
Human PBMCs were plated in 96-well plates (left) or 384-well plates (right) at 40,000 cells/well or 10,000 cells/well, respectively, and treated with a cell stimulation cocktail to induce IL-2 release. The Lumit™ IL-2 Immunoassay reagents were added directly to the cell culture wells. The calculated EC50 values are similar in the 96-well plate and 384-well plate formats.
Explore Additional Cytokine Detection Assays
We offer a growing portfolio of assays to detect cytokines in cell culture samples.
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For a biotech that understands time is of significant value, the Lumit™ Immunoassays are an essential way for us to gather reliable data fast so that we can reach conclusions and make decisions sooner.
Jonathan Chow, Principal Scientist, Corner Therapeutics