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홈 > 제품정보 > 시약 > 국내 공식 대리점 > Lumit™ Human IL-1β Immunoassay, 100 assays
Lumit™ Human IL-1β Immunoassay, 100 assays
제품코드 : W6010
제품정보 : Inflammation Assay
- Lumit Immunoassay는 washing, transfer, sample dilution 단계없이 세포 배양 상태에서 바로 analyte를 특정할 수 있는 새로운 immunoassay입니다.
- [W6010] 100 assays, [W6012] 500 assays, [W6011] 1,000 assays
제품문의
-
- 전화번호
- 2292-8870
- 이메일
- dreamcell@dreamcell.co.kr
제품상세
No-Wash Immunoassay for IL-1β Detection in Cells
- Fast add-and-read protocol performed directly on cells
- Luminescent detection with just a standard plate-reading luminometer
- Scalable to 96- or 384-well plates
Quantify Inflammasome Activation Without Tedious Wash Steps
The Lumit™ IL-1β Immunoassay quantitatively measures released IL-1β in cell culture samples using a simple, no-wash protocol. Just add labeled antibodies to the sample, add detection reagent and read luminescent signal using a standard plate-reading luminometer. The entire protocol is completed in less than 70 minutes! The assay can be used directly on cells in culture or culture medium transferred to a separate assay plate.
How the Lumit™ IL-1β Immunoassay Works
Primary antibodies to IL-1β were selected for their specific and sensitive detection and labeled with the LgBiT and SmBiT subunits of NanoBiT® Luciferase. In the presence of IL-1β, the subunits are brought together to form an active luciferase enzyme. Addition of optimized substrate generates a bright luminescent signal proportional to IL-1β levels.
Simple One-Plate Protocol, No Wash Steps!
Large Dynamic Range of Lumit™ Detection
The picomolar to nanomolar linear range of Lumit™ detection covers expected concentrations for most cell models.
Assay | IL-1β (Human) Immunoassay | IL-1β (Mouse) Immunoassay |
Limit of Detection (LOD) | 10pg/ml (3 SD above background) | 8pg/ml (3 SD above background) |
Dynamic Range | 22pg/ml – 40ng/ml | 11pg/ml – 20ng/ml |
Minimal Detectable Dose (MDD) | 7pg/ml (2 SD above background) | 5pg/ml (2 SD above background) |
Assay Time | 70 minutes | |
Sample Type | Cell culture supernatants | |
SD=Standard Deviation
High-Throughput NLRP3 Inflammasome Inhibitor Screening
The add-and-read format of the Lumit™ IL-1β Immunoassay enables high-throughput screening for modulators of IL-1β release.
The NLRP3 inflammasome inhibitor, MCC950, prevents caspase-1 activation and release of mature IL-1β. A titration of MCC950 was added to differentiated THP-1 cells (5 × 104/well) followed by LPS (20 EU/ml) or vehicle. The Lumit™ IL-1β (Human) Immunoassay was performed after 5 hours.