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홈 > 제품정보 > 시약 > 국내 공식 대리점 > Caspase-Glo® 3/7 Assay, 2.5ml
Caspase-Glo® 3/7 Assay, 2.5ml
제품코드 : G8090
제품정보 : Apoptosis Assay
- 간단한 실험 방법으로 caspase 활성을 감도 높게 측정할 수 있는 발광 기반의 제품입니다.
- [G8090] 2.5ml, [G8091] 10ml, [G8092] 100ml, [G8093] 10 X10ml
제품문의
-
- 전화번호
- 2292-8870
- 이메일
- dreamcell@dreamcell.co.kr
제품상세
Caspase Activity Assay for Apoptosis Detection
- High sensitivity caspase assay—requires fewer cells and less enzyme
- Simple add-mix-measure protocol—no sample preparation
- Easily scalable to 96-, 384- and 1,536-well plate formats
Homogeneous Assay Measures Caspase-3/7 Activity
The Caspase-Glo® 3/7 assay provides a proluminescent caspase-3/7 DEVD-aminoluciferin substrate and a proprietary thermostable luciferase in a reagent optimized for caspase-3/7 activity, luciferase activity and cell lysis. Adding the single Caspase-Glo® 3/7 Reagent in an "add-mix-measure" format results in cell lysis, followed by caspase cleavage of the substrate. This liberates free aminoluciferin, which is consumed by the luciferase, generating a "glow-type" luminescent signal that is proportional to caspase-3/7 activity.
The stabilized luciferase and proprietary buffer system improve assay performance across a wide range of assay conditions, and the assay is less likely to be affected by compound interference than fluorescent- or colorimetric-based assays. The caspase activity assay is designed for use with multiwell plate formats using either purified enzyme or cells in culture and can be multiplexed with other cell-based assays. The assay delivers excellent Z´-factor values in cell and purified enzyme models.
Simple Bioluminescent Detection of Caspase Activity
Luminescence Is Linear Over a Broad Range of Cell Numbers
Jurkat cells were treated with anti-Fas mAb for 4.5 hours to induce apoptosis or were left untreated. Caspase-Glo® 3/7 Reagent was added directly to the cells in 96-well plates and incubated for 1 hour before recording luminescence. Each point represents the average of 4 wells. The "no cell" blank control value has been subtracted from each.
Multiplex Caspase 3/7 with Cytotoxicity and Cell Viability Assays
Notes
Using 100μl of Caspase-Glo® Reagent per assay in a 96-well format, Cat.# G8090 provides sufficient reagents to perform 25 caspase activity assays; Cat.# G8091, 100 assays; Cat.# G8092 and G8093, 1,000 assays. Using 25μl of Caspase-Glo® Reagent per assay in a 384-well format, Cat.# G8090 provides sufficient reagents to perform 100 caspase activity assays; Cat.# G8091, 400 assays; Cat.# G8092 and G8093, 4,000 assays.