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홈 > 제품정보 > 시약 > 국내 공식 대리점 > CellTox™ Green Cytotoxicity Assay, 10ml
CellTox™ Green Cytotoxicity Assay, 10ml
제품코드 : G8741
제품정보 : Cytotoxicity Assay
- DNA를 염색하는 형광 dye 기법으로 최대 72시간까지 세포 독성 변화 모니터링이 가능합니다.
- Real-time cell death assay
- [G8741] 10ml, [G8742] 50ml, [G8743] 100ml
- [G8731] 200ul (Express Assay)
제품문의
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- 전화번호
- 2292-8870
- 이메일
- dreamcell@dreamcell.co.kr
제품상세
Real-Time Cell Death Assay With Multiplexing Compatibility
- Accurate cytotoxicity determination in exposures out to 72 hours
- Flexible protocol allows kinetic analysis or endpoint determination
- Multiplex with luminescent assays to obtain more data per well
Monitoring Cell Death in Real Time Using the CellTox™ Green Cytotoxicity Assay
Cytotoxicity assays based on detection of biomarkers released into the media can underestimate cytotoxicity in long-term exposures of 72 hours or more because of limited stability of the biomarker detected. The CellTox™ Green Cytotoxicity Assay provides an easy, fast and accurate method to determine toxic effects during or after long-term exposure of cells in culture. CellTox Green can be combined with other methods in multiplex assays to determine mechanism of toxicity, and is easily scalable from 96- to 1536-well plate formats.
CellTox™ Green Dye binds DNA of cells with impaired membrane integrity.
Perform Kinetic Cytotoxicity Measures to Determine Onset of Toxicity
Dose- and exposure-dependent increases in cytotoxicity
In the experiment shown here, bortezomib caused appreciable cytotoxicity in K562 cells between the 4- and 24-hour time points. Additive fluorescence at later time points indicates continued loss of membrane integrity occurring as a function of cytotoxicity against remaining cells. The CellTox™ Green Dye was added at seeding.
Multiplex with Cell Viability Assays for More Informative Data per Well
Measure Cytotoxicity and Viability
Here, CellTox™ Green Dye was mixed with K562 Cells, which were plated, then dosed with compound. CellTiter-Glo® Cell Viability Assay Reagent was added at the end of the 72-hour exposure and luminescence (viability) measured. These two inverse measures of cell health resulted in EC50 agreement.
Determine Appropriate Timing of Caspase Assays
CellTox™ Green can be used in kinetic mode to determine the onset of cytotoxicity followed by multiplexing with the Caspase-Glo® 3/7 Assay in the same sample well to confirm caspase-3/7 activation at the appropriate time.
Differentiate Between Cytotoxic and Cytostatic Effects
In this example, K562 cells were exposed to Methotrexate, an anti-proliferative compound, for 72 hours. CellTox™ Green Dye was added to determine cytotoxicity, then CellTiter-Glo® Reagent was added to the same sample well for viability determination. A viability assay alone would have misreported the compound as cytotoxic due to less cells in the treated sample compared to the non-treated control, which continued to proliferate. Multiplexing with a cytotoxicity assay reveals no change in signal as a result of compromised membrane integrity, indicating growth inhibition and not cytotoxicity.
