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A Luminescent Cell Viability Assay for Fast, Easy, Everyday Use

• ATP assay for detection of viable cells
• Reduced preparation time and convenient repeat use over multiple days
• Same great performance as classic CellTiter-Glo® Cell Viability Assay
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CellTiter-Glo® 2.0 Measures ATP, a Key Indicator of Cell Health

CellTiter-Glo® 2.0, based on the original CellTiter-Glo® Cell Viability Assay chemistry, is a single-reagent assay with improved stability for convenient storage and simpler setup. Whether you perform cell proliferation assays in a single plate or process hundreds of plates at a time, the ready-to-use reagent is designed for fast and easy everyday use. A highly sensitive ATP assay with broad linearity, it is amenable to high- throughput screening and multiplex applications.

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Simple Assay Setup and Enhanced Stability

A single, ready-to-use reagent and simple "add-mix-measure" protocol mean there's no need to mix components.

The CellTiter-Glo® 2.0 reagent is stable at room temperature and at 4°C, allowing fast and easy assay implementation, and eliminating the thawing and mixing required with other ATP assays. Enhanced stability also allows convenient storage.

Percent Activity Remaining	CellTiter-Glo® 2.0 Reagent Stability	CellTiter-Glo® "Original" Reagent Stability
>85% Activity at 22°C	7 days	12 hours
>85% Activity at 4°C	2 months	3.5 days

Assay Performance

The CellTiter-Glo® 2.0 Cell Viability Assay exhibits the same high performance as the classic CellTiter-Glo® Assay, making it suitable for use from benchtop scale to high-throughput screening.

High sensitivity and broad linearity. Luminescence measured with the CellTiter-Glo® 2.0 Assay is proportional to the number of viable Jurkat cells in culture over three orders of magnitude in 96-well, 384-well and 1536-well plates.

Multiplex With Other Cell Viability or Cytotoxicity Assays

CellTiter-Glo® 2.0 cell viability assay can be multiplexed with other assays, reducing cell culture costs and labor. Multiplexing assays in the same sample well allows complementary measures of cell health to correct for errors in culturing, plating or assay chemistry interferences.

In the example shown here, CellTox™ Green cytotoxicity assay reagent was added to bortezemib-treated K562 cells after 48 hours of exposure. Fluorescence associated with cytotoxicity was measured. The CellTiter-Glo® 2.0 assay reagent was added and luminescence measured as an indicator of cell proliferation.

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CellTiter-Glo(R) 2.0 Assay Technical Manual TM403