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> 제품정보 > 시약 > 해외 공식 대리점 > [C-28075] 3D Tumorsphere Medium XF
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[C-28075] 3D Tumorsphere Medium XF

제품코드 : C-28075

제품정보 :

  • 3D Tumorsphere Medium XF (Ready-to-use), phenol red-free 250 ml

제품문의

  • 전화번호
    2292-8870

제품상세

Key benefits:

  • Establish 3D tumorsphere cultures directly from 2D cancer cell lines
  • Suitable for long-term routine culture of tumorspheres
  • Compatible with most commonly used cancer cell lines
  • Xeno-free and serum-free formulation

Components:

  • 3D Tumorsphere Basal Medium
  • 3D Tumorsphere SupplementMix

3D cancer spheroids and organoids are popular in vitro models that often better reflect the in vivo tumor environment than some 2D models. Within tumors and tumor spheroids, cancer stem cells (CSCs) are a small subpopulation of cells that play a key role in tumor initiation, recurrence, metastasis, and even resistance to cancer therapies. CSCs exhibit stem cell-like characteristics including self-renewal capabilities and tumorigenicity.

As a part of our Cancer Media Toolbox, our 3D Tumorsphere Medium XF is ideal for isolation and 3D long-term cultivation of CSCs. 3D Tumorsphere Medium XF supports enrichment and maintenance of cancer stem cells as well as further differentiated cancer cells with high cell proliferation rates.

The medium helps you create a reducible in vivo-like system by promoting the formation of homogenous tumorspheres through serial passaging of the 3D culture.

When it comes to the characterization of cultured CSCs, the tumorsphere formation efficiency (TFE) assay represents the gold standard. The TFE assay describes the percentage of cells within a cancer cell culture that can form a sphere from a single cell. Since only stem-like cells can form spheroids from a single cell, TFE values are a quantitative measurement that correlates with cancer metastasis and aggressiveness. In experiment where MCF-7 cells have been cultured in 3D Tumorsphere Medium XF, the TFE value significantly improved from 2% to 28% over 9 passages.

3D Tumorsphere Medium XF In Cancer Media Tool Box

The 3D Tumorsphere Medium XF is flexible enough to support different cell types tested for serial passage:

TissueTested cell linesCell lines origin
BrainU-87 MGGrade IV glioblastoma / astrocytoma of the human brain
BreastMCF-7Pleural effusion of metastatic human breast adenocarcinoma
BreastMDA-MB-231Pleural effusion of metastatic human breast adenocarcinoma (triple-negative)
ColonHT-29Human colon adenocarcinoma
Connective tissueHT1080human fibrosarcoma
LiverHepG2Hepatocellular carcinoma of the human liver
LungA-549Human lung carcinoma
PancreasPanc-1Epithelioid carcinoma of the human pancreatic duct
ProstateLNCaPLymph node metastasis of human prostate adenocarcinoma
SkinA-431Epidermoid carcinoma of the human skin
ColonCT26Mouse colon cancer cell line