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홈 > 제품정보 > 시약 > 해외 공식 대리점 > [CSB-EL004925HU] Human Myeloid cell surface antigen CD33(CD33) ELISA kit
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[CSB-EL004925HU] Human Myeloid cell surface antigen CD33(CD33) ELISA kit

제품코드 : CSB-EL004925HU

제품정보 : Human Myeloid cell surface antigen CD33(CD33)

  • Sample Types: serum, plasma, tissue homogenates
  • Detect Range: 125 pg/mL-8000 pg/mL
  • Sensitivity: 31.25 pg/mL

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  • 전화번호
    2292-8870

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Product Details

Description

This Human CD33 ELISA Kit was designed for the quantitative measurement of Human CD33 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 125 pg/mL-8000 pg/mL and the sensitivity is 31.25 pg/mL .

Target NameCD33 molecule
Alternative NamesCD33; SIGLEC3; Myeloid cell surface antigen CD33; Sialic acid-binding Ig-like lectin 3; Siglec-3; gp67; CD antigen CD33
AbbreviationCD33
Uniprot No.P20138
SpeciesHomo sapiens (Human)
Sample Typesserum, plasma, tissue homogenates
Detection Range125 pg/mL-8000 pg/mL
Sensitivity31.25 pg/mL
Assay Time1-5h
Sample Volume50-100ul
Detection Wavelength450 nm
Research AreaImmunology
Assay Principlequantitative
MeasurementSandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%   
Three samples of known concentration were tested twenty times on one plate to assess. 
Inter-assay Precision (Precision between assays): CV%<10%   
Three samples of known concentration were tested in twenty assays to assess.  
       
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CD33 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　SampleSerum(n=4) 
1:1Average %89 
Range %80-101 
1:2Average %97 
Range %91-104 
1:4Average %98 
Range %90-113 
1:8Average %94 
Range %85-100 
Recovery
The recovery of human CD33 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange 
Serum (n=5)9689-104 
EDTA plasma (n=4)9590-102 
       
       
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected 
80002.1302.1742.1521.993 
40001.7321.7541.7431.584 
20001.3341.3551.3451.186 
10000.8260.8500.8380.679 
5000.5650.5800.5730.414 
2500.4200.4170.4190.260 
1250.3020.2950.2990.140 
00.1540.1640.159　 
ELISA Data AnalysisWatch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs		ELISA kit FAQs
StorageStore at 2-8°C. Please refer to protocol.
Lead Time3-5 working days

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