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[CSB-EL004943HU] Human CAMPATH-1 antigen(CD52) ELISA kit

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홈 > 제품정보 > 시약 > 해외 공식 대리점 > [CSB-EL004943HU] Human CAMPATH-1 antigen(CD52) ELISA kit

제품코드 : CSB-EL004943HU

제품정보 : Human CAMPATH-1 antigen(CD52)

Sample Types: serum, plasma, tissue homogenates, cell lysates
Detect Range: 78 ng/mL-5000 ng/mL
Sensitivity: 19.5 ng/mL

전화번호

2292-8870

이메일

dreamcell@dreamcell.co.kr

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Description

This Human CD52 ELISA Kit was designed for the quantitative measurement of Human CD52 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 78 ng/mL-5000 ng/mL and the sensitivity is 19.5 ng/mL .

Target Name

CD52 molecule

Alternative Names

Cambridge pathology 1 antigen ELISA Kit; CAMPATH 1 ELISA Kit; CAMPATH 1 antigen ELISA Kit; CAMPATH-1 antigen ELISA Kit; CD 52 ELISA Kit; CD52 ELISA Kit; CD52 antigen ELISA Kit; CD52 molecule ELISA Kit; CD52_HUMAN ELISA Kit; CDw52 ELISA Kit; CDW52 antigen ELISA Kit; Epididymal secretory protein E5 ELISA Kit; Epididymis secretory sperm binding protein Li 171mP ELISA Kit; HE 5 ELISA Kit; He5 ELISA Kit; Human epididymis-specific protein 5 ELISA Kit

Abbreviation

CD52

Uniprot No.

P31358

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates, cell lysates

Detection Range

78 ng/mL-5000 ng/mL

Sensitivity

19.5 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CD52 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-90
1:2	Average %	98
	Range %	91-105
1:4	Average %	96
	Range %	92-100
1:8	Average %	96
	Range %	89-102

Recovery

The recovery of human CD52 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	80-100
EDTA plasma (n=4)	98	91-110

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
5000	1.975	1.952	1.964	1.870
2500	1.612	1.586	1.599	1.505
1250	1.272	1.171	1.222	1.128
625	0.984	0.932	0.958	0.864
312	0.665	0.629	0.647	0.553
156	0.398	0.368	0.383	0.289
78	0.193	0.201	0.197	0.103
0	0.097	0.091	0.094