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> 제품정보 > 시약 > 해외 공식 대리점 > [CSB-EL011657HU] Human Interleukin-34(IL34) ELISA kit
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[CSB-EL011657HU] Human Interleukin-34(IL34) ELISA kit

제품코드 : CSB-EL011657HU

제품정보 : Human Interleukin-34(IL34)

  • Sample Types: serum, plasma, tissue homogenates, cell culture supernates
  • Detect Range: 23.5 pg/mL-1500 pg/mL
  • Sensitivity: 5.86 pg/mL

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  • 전화번호
    2292-8870

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Product Details

Description

This Human IL34 ELISA Kit was designed for the quantitative measurement of Human IL34 protein in serum, plasma, tissue homogenates, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.86 pg/mL.

Target Nameinterleukin 34
Alternative NamesC16orf77 ELISA Kit; Chromosome 16 open reading frame 77 ELISA Kit; FPT025 ELISA Kit; IL 34 ELISA Kit; IL-34 ELISA Kit; Il34 ELISA Kit; IL34_HUMAN ELISA Kit; Interleukin 34 ELISA Kit; Interleukin-34 ELISA Kit; Interleukin34 ELISA Kit; MGC34647 ELISA Kit
AbbreviationIL34
Uniprot No.Q6ZMJ4
SpeciesHomo sapiens (Human)
Sample Typesserum, plasma, tissue homogenates, cell culture supernates
Detection Range23.5 pg/mL-1500 pg/mL
Sensitivity5.86 pg/mL
Assay Time1-5h
Sample Volume50-100ul
Detection Wavelength450 nm
Research AreaImmunology
Assay Principlequantitative
MeasurementSandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%    
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%    
Three samples of known concentration were tested in twenty assays to assess.   
        
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human IL34 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. 
 SampleSerum(n=4)  
1:1Average %93  
Range %89-97  
1:2Average %85  
Range %80-90  
1:4Average %104  
Range %100-108  
1:8Average %98  
Range %94-102  
Recovery
The recovery of human IL34 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. 
 
Sample TypeAverage % RecoveryRange  
Serum (n=5)8984-93  
EDTA plasma (n=4)9288-96  
        
        
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
 
pg/mlOD1OD2AverageCorrected  
15002.7562.8202.7882.696  
7502.2482.1462.1972.105  
3751.5371.6011.5691.477  
187.50.9120.9550.9340.842  
93.750.4910.4680.4800.388  
46.880.2740.2820.2780.186  
23.440.1850.1790.1820.090  
00.0890.0940.092   
ELISA Data AnalysisWatch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
StorageStore at 2-8°C. Please refer to protocol.
Lead Time3-5 working days