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> 제품정보 > 시약 > 해외 공식 대리점 > [CSB-E07436r] Rat Interleukin 3,IL-3 ELISA KIT
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[CSB-E07436r] Rat Interleukin 3,IL-3 ELISA KIT

제품코드 : CSB-E07436r

제품정보 : Rat Interleukin 3,IL-3

  • Sample Types: serum, plasma, tissue homogenates
  • Detect Range: 3.12 pg/mL-200 pg/mL
  • Sensitivity: 0.78 pg/mL

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  • 전화번호
    2292-8870

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Product Details

Description

This Rat IL3 ELISA Kit was designed for the quantitative measurement of Rat IL3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL.

Target Nameinterleukin 3 (colony-stimulating factor, multiple)
Alternative NamesIl3 ELISA Kit; Il-3Interleukin-3 ELISA Kit; IL-3 ELISA Kit; Hematopoietic growth factor ELISA Kit; Mast cell growth factor ELISA Kit; MCGF ELISA Kit; Multipotential colony-stimulating factor ELISA Kit; P-cell-stimulating factor ELISA Kit
AbbreviationIL3
Uniprot No.P04823
SpeciesRattus norvegicus (Rat)
Sample Typesserum, plasma, tissue homogenates
Detection Range3.12 pg/mL-200 pg/mL
Sensitivity0.78 pg/mL
Assay Time1-5h
Sample Volume50-100ul
Detection Wavelength450 nm
Research AreaImmunology
Assay Principlequantitative
MeasurementSandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%   
Three samples of known concentration were tested twenty times on one plate to assess. 
Inter-assay Precision (Precision between assays): CV%<10%   
Three samples of known concentration were tested in twenty assays to assess.  
       
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4) 
1:1Average %94 
Range %90-98 
1:2Average %100 
Range %95-113 
1:4Average %91 
Range %85-97 
1:8Average %97 
Range %92-104 
Recovery
The recovery of rat IL-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange 
Serum (n=5)9085-94 
EDTA plasma (n=4)9387-98 
       
       
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected 
2002.4362.2762.3562.214 
1001.9752.0141.9951.853 
501.4351.3721.4041.262 
250.8680.9130.8910.749 
12.50.5530.5190.5360.394 
6.250.3870.4070.3970.255 
3.120.2850.2760.2810.139 
00.1440.1400.142  
ELISA Data AnalysisWatch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
StorageStore at 2-8°C. Please refer to protocol.
Lead Time3-5 working days