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> 제품정보 > 시약 > 해외 공식 대리점 > [CSB-E04632m] Mouse Interleukin 3,IL-3 ELISA KIT
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[CSB-E04632m] Mouse Interleukin 3,IL-3 ELISA KIT

제품코드 : CSB-E04632m

제품정보 : Mouse Interleukin 3,IL-3

  • Sample Types: serum, plasma, tissue homogenates
  • Detect Range: 7.8 pg/mL-500 pg/mL
  • Sensitivity: 1.95 pg/mL

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  • 전화번호
    2292-8870

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Product Details

Description

This Mouse IL3 ELISA Kit was designed for the quantitative measurement of Mouse IL3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL.

Target Nameinterleukin 3 (colony-stimulating factor, multiple)
Alternative NamesIl3 ELISA Kit; Csfmu ELISA Kit; Il-3 ELISA Kit; Interleukin-3 ELISA Kit; IL-3 ELISA Kit; Hematopoietic growth factor ELISA Kit; Mast cell growth factor ELISA Kit; MCGF ELISA Kit; Multipotential colony-stimulating factor ELISA Kit; P-cell-stimulating factor ELISA Kit
AbbreviationIL3
Uniprot No.P01586
SpeciesMus musculus (Mouse)
Sample Typesserum, plasma, tissue homogenates
Detection Range7.8 pg/mL-500 pg/mL
Sensitivity1.95 pg/mL
Assay Time1-5h
Sample Volume50-100ul
Detection Wavelength450 nm
Research AreaImmunology
Assay Principlequantitative
MeasurementSandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %88
Range %83-92
1:2Average %95
Range %91-100
1:4Average %90
Range %86-99
1:8Average %95
Range %86-98
Recovery
The recovery of mouse IL-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5)9490-98
EDTA plasma (n=4)9786-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
5002.6952.6802.6882.511
2501.8851.8671.8761.699
1251.3021.2951.2991.122
62.50.7580.7440.7510.574
31.20.4990.4920.4960.319
15.60.3560.3590.3580.181
7.80.2810.2870.2840.107
00.1750.1790.177 
ELISA Data AnalysisWatch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
StorageStore at 2-8°C. Please refer to protocol.
Lead Time3-5 working days

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