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> 제품정보 > 시약 > 해외 공식 대리점 > [CSB-E06881r] Rat prolactin,PRL ELISA Kit
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[CSB-E06881r] Rat prolactin,PRL ELISA Kit

제품코드 : CSB-E06881r

제품정보 : Rat prolactin,PRL

  • Sample Types: serum, plasma, cell culture supernates, tissue homogenates, cell lysates
  • Detect Range: 0.125 ng/mL-50 ng/mL
  • Sensitivity: 0.125 ng/mL

제품문의

  • 전화번호
    2292-8870

제품상세

Product Details

Description

This Rat PRL ELISA Kit was designed for the quantitative measurement of Rat PRL protein in serum, plasma, cell culture supernates, tissue homogenates, cell lysates. It is a Competitive ELISA kit, its detection range is 0.125 ng/mL-50 ng/mL and the sensitivity is 0.125 ng/mL.

Target Nameprolactin
Alternative NamesPrl ELISA Kit; Prolactin ELISA Kit; PRL ELISA Kit
AbbreviationPRL
Uniprot No.P01237
SpeciesRattus norvegicus (Rat)
Sample Typesserum, plasma, cell culture supernates, tissue homogenates, cell lysates
Detection Range0.125 ng/mL-50 ng/mL
Sensitivity0.125 ng/mL
Assay Time1-5h
Sample Volume50-100ul
Detection Wavelength450 nm
Research AreaSignal Transduction
Assay Principlequantitative
MeasurementCompetitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%   
Three samples of known concentration were tested twenty times on one plate to assess. 
Inter-assay Precision (Precision between assays): CV%<15%   
Three samples of known concentration were tested in twenty assays to assess.  
       
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat PRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4) 
1:1Average %95 
Range %90-105 
1:2Average %94 
Range %91-105 
1:4Average %95 
Range %90-101 
1:8Average %97 
Range %92-104 
Recovery
The recovery of rat PRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange 
Serum (n=5)9287-97 
EDTA plasma (n=4)9790-105 
       
       
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2Average  
500.1470.1460.147  
9.370.3050.3140.310  
1.560.5120.5230.518  
0.440.8650.8870.876  
0.1251.4111.4241.418  
02.1182.1222.120  
ELISA Data AnalysisWatch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
StorageStore at 2-8°C. Please refer to protocol.
Lead Time3-5 working days

Citations