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[CSB-E06883h] Human Prolactin/Luteotropic Hormone(PRL/LTH) ELISA Kit

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홈 > 제품정보 > 시약 > 해외 공식 대리점 > [CSB-E06883h] Human Prolactin/Luteotropic Hormone(PRL/LTH) ELISA Kit

제품코드 : CSB-E06883h

제품정보 : Human Prolactin/Luteotropic Hormone(PRL/LTH)

Sample Types: serum, plasma, cell culture supernates, tissue homogenates, cell lysates
Detect Range: 40 μIU/mL-2000 μIU/mL
Sensitivity: 50 μIU/mL

전화번호

2292-8870

이메일

dreamcell@dreamcell.co.kr

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

Description

This Human PRL ELISA Kit was designed for the quantitative measurement of Human PRL protein in serum, plasma, cell culture supernates, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 40 μIU/mL-2000 μIU/mL and the sensitivity is 50 μIU/mL.

Target Name

prolactin

Alternative Names

PRL ELISA Kit; Prolactin ELISA Kit; PRL ELISA Kit

Abbreviation

PRL

Uniprot No.

P01236

Species

Homo sapiens (Human)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates, cell lysates

Detection Range

40 μIU/mL-2000 μIU/mL

Sensitivity

50 μIU/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human PRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	86-97
1:2	Average %	92
	Range %	87-100
1:4	Average %	95
	Range %	92-99
1:8	Average %	95
	Range %	90-98

Recovery

The recovery of human PRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-102
EDTA plasma (n=4)	97	92-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

μIU/ml	OD1	OD2	Average	Corrected
2000	2.186	2.105	2.146	2.115
800	1.201	1.197	1.199	1.168
300	0.622	0.619	0.621	0.590
100	0.296	0.302	0.299	0.268
40	0.145	0.148	0.147	0.116
0	0.032	0.030	0.031