| Description | This Mouse estrogen (E) ELISA Kit was designed for the quantitative measurement of Mouse estrogen (E) protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 25 pg/mL-1000 pg/mL and the sensitivity is 25 pg/mL. |
| Alternative Names | N/A |
| Abbreviation | E |
| Species | Mus musculus (Mouse) |
| Sample Types | serum, plasma |
| Detection Range | 25 pg/mL-1000 pg/mL |
| Sensitivity | 25 pg/mL |
| Assay Time | 1-5h |
| Sample Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Signal Transduction
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| Assay Principle | quantitative |
| Measurement | Competitive |
| Precision | | Intra-assay Precision (Precision within an assay): CV%<15% | | | | | Three samples of known concentration were tested twenty times on one plate to assess. | | | Inter-assay Precision (Precision between assays): CV%<15% | | | | | Three samples of known concentration were tested in twenty assays to assess. | | | | | | | | | | |
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| Linearity | | To assess the linearity of the assay, samples were spiked with high concentrations of mouse estrogen in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | | | Sample | Serum(n=4) | | | 1:1 | Average % | 95 | | | Range % | 90-100 | | | 1:2 | Average % | 92 | | | Range % | 86-98 | | | 1:4 | Average % | 98 | | | Range % | 95-105 | | | 1:8 | Average % | 90 | | | Range % | 88-92 | |
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| Recovery | | The recovery of mouse estrogen spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
| Sample Type | Average % Recovery | Range | | | Serum (n=5) | 94 | 90-98 | | | EDTA plasma (n=4) | 96 | 91-100 | | | | | | | | | | | | | | | | | |
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| Typical Data | | These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
 | pg/ml | OD1 | OD2 | Average | | | | 0 | 2.411 | 2.397 | 2.404 | | | | 25 | 1.485 | 1.526 | 1.506 | | | | 75 | 0.900 | 0.858 | 0.879 | | | | 230 | 0.564 | 0.577 | 0.571 | | | | 500 | 0.386 | 0.364 | 0.375 | | | | 1000 | 0.182 | 0.183 | 0.183 | | |
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| Materials provided | - A 96-well Assay plate--The 96-well plate has been pre-coated with goat-anti-rabbit estrogen antibody.
- Standard (6 x 0.5 ml)--Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
- HRP-conjugated estrogen(1 x 6 ml) --Bind to the anti-mouse estrogen antibody, and HRP catalyzes the TMB to elicit a chromogenic reaction.
- Anti-mouse estrogen Antibody (1 x 6 ml)--React with both estrogen in the samples/standards and HRP-conjugated estrogen.
- Wash Buffer (20x concentrate) 1 x 15ml --Wash away unbound or free substances.
- Substrate A (1 x 7 ml) --Mix with Substrate B and interacts with HRP, eliciting the solution turns blue.
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| Materials not provided | - A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
- An incubator that can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
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| ELISA Data Analysis | Watch ELISA data processing video & download Curve Expert if needed |
Troubleshooting
and FAQs | ELISA kit FAQs |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Lead Time | 3-5 working days |
